Mast cell stabilization promotes antinociceptive effects in a mouse model of postoperative pain.
Mast cell stabilization promotes antinociceptive effects in a mouse model of postoperative pain.
复制标题
DOI：
10.2147/jpr.s41527
复制
发表时间：
2013
影响因子：
2.7
通讯作者：
Masaki E
中科院分区：
文献类型：
--
作者：
Yasuda M;Kido K;Ohtani N;Masaki E
神经损伤和随之而来的手术切口产生的炎症反应导致复杂的疼痛状态，仍然影响着一半的手术患者。因此，麻醉师必须确定术后疼痛的机制。肥大细胞是结缔组织和粘膜的常驻细胞，参与免疫反应。肥大细胞的脱颗粒与术后疼痛的发生有关，可由手术切口引起。本研究的目的是研究肥大细胞的稳定是否会在小鼠术后疼痛模型中引起抗伤害感受作用。术后在BALB/c小鼠后爪处切开引起疼痛。在足部切开前注射柱细胞膜稳定剂cromoglycate (200 μg/20 μL)，并在切开后1、2、7天通过评估保护行为、机械刺激戒断阈值和热痛行为潜伏期来测量术后疼痛反应。切口产生保护性疼痛、机械异常性痛和热过敏。cromoglyate降低了保护性疼痛评分（第1天）和对机械刺激的戒断阈值（第1、2和7天）。然而，对热的戒断潜伏期不受甘糖酸处理的影响。cromoglyate显著减轻了小鼠术后疼痛模型中表现为保护性疼痛和机械异常性疼痛的疼痛反应。因此，肥大细胞活化可能是术后疼痛的一种机制，也是开发新疗法的一个有趣目标。
Nerve injury and consequent inflammatory responses produced by surgical incision result in a complicated pain status which still affects half of all surgical patients. Therefore, it is essential for anesthesiologists to identify the mechanisms of postoperative pain. Mast cells are resident cells of connective tissue and the mucosa that participate in the immune response. Degranulation of mast cells is involved in the development of postoperative pain and can be induced by surgical incision. The aim of this study was to investigate whether stabilization of mast cells causes an antinociceptive effect in a mouse model of postoperative pain. Postoperative pain was induced by making an incision in the hind paw of BALB/c mice. The mast cell membrane stabilizer cromoglycate (200 μg/20 μL) was injected before incision of the paw, and postoperative pain responses were measured by assessing guarding behavior, withdrawal threshold to mechanical stimuli, and latency of heat pain behavior 1, 2, and 7 days after the incision. The incision produced guarding pain, mechanical allodynia, and heat hypersensitivity. Cromoglycate decreased the guarding pain score (day 1) and the withdrawal threshold to mechanical stimuli (days 1, 2, and 7). However, the withdrawal latency to heat was not affected by cromoglycate treatment. Cromoglycate significantly attenuated the pain response expressed as guarding pain and mechanical allodynia in a mouse model of postoperative pain. Thus, mast cell activation is likely a mechanism of postoperative pain and is an interesting target for the development of new therapies.