A mutation in THERMOSENSITIVE MALE STERILE 1, encoding a heat shock protein with DnaJ and PDI domains, leads to thermosensitive gametophytic male sterility in Arabidopsis
A mutation in THERMOSENSITIVE MALE STERILE 1, encoding a heat shock protein with DnaJ and PDI domains, leads to thermosensitive gametophytic male sterility in Arabidopsis
复制标题
编码具有 DnaJ 和 PDI 结构域的热休克蛋白的热敏雄性不育 1 的突变导致拟南芥中热敏配子体雄性不育
DOI：
10.1111/j.1365-313x.2008.03732.x
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发表时间：
2009-03-01
期刊：
影响因子：
7.2
通讯作者：
Ye, De
中科院分区：
文献类型：
--
作者：
Yang, Ke-Zhen;Xia, Chuan;Ye, De
在大多数开花植物物种中，授粉和受精发生在炎热的夏季，因此植物必须进化出一种机制，以确保其花粉管在高温下正常生长。尽管它对植物繁殖的重要性，很少有人知道的分子基础的耐热性在花粉管。在这里，我们报告了一个新的拟南芥基因，温敏雄性不育1（TMS 1），这在花粉管的耐热性起着重要作用的鉴定和特征。TMS 1编码Hsp40同源蛋白，其具有在蛋白质二硫键异构酶（PDI）中发现的DnaJ结构域和a_ERdj5_C结构域。在大肠杆菌（BL21 DE3）中表达的TMS 1具有还原PDI的活性。TMS 1在花粉粒、花粉管和其它营养组织中表达，包括叶、茎和根。37 ℃热激处理增加了其在生长中的花粉管以及营养组织中的表达水平。在30摄氏度下生长的TMS 1中的敲除突变极大地延缓了传输道中的花粉管生长，导致雄性育性显著降低。我们的研究表明，TMS 1所需的耐热性在拟南芥中的花粉管，可能是作为一个共同的分子伴侣。
In most flowering plant species, pollination and fertilization occur during the hot summer, so plants must have evolved a mechanism that ensures normal growth of their pollen tubes at high temperatures. Despite its importance to plant reproduction, little is known about the molecular basis of thermotolerance in pollen tubes. Here we report the identification and characterization of a novel Arabidopsis gene, THERMOSENSITIVE MALE STERILE 1 (TMS1), which plays an important role in thermotolerance of pollen tubes. TMS1 encodes a Hsp40-homologous protein with a DnaJ domain and an a_ERdj5_C domain found in protein disulfide isomerases (PDI). Purified TMS1 expressed in Escherichia coli (BL21 DE3) had the reductive activity of PDI. TMS1 was expressed in pollen grains, pollen tubes and other vegetative tissues, including leaves, stems and roots. Heat shock treatment at 37 degrees C increased its expression levels in growing pollen tubes as well as in vegetative tissues. A knockout mutation in TMS1 grown at 30 degrees C had greatly retarded pollen tube growth in the transmitting tract, resulting in a significant reduction in male fertility. Our study suggests that TMS1 is required for thermotolerance of pollen tubes in Arabidopsis, possibly by functioning as a co-molecular chaperone.