Arabidopsis REM16 acts as a B3 domain transcription factor to promote flowering time via directly binding to the promoters of SOC1 and FT
Arabidopsis REM16 acts as a B3 domain transcription factor to promote flowering time via directly binding to the promoters of SOC1 and FT
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拟南芥 REM16 作为 B3 结构域转录因子，通过直接结合 SOC1 和 FT 的启动子来促进开花时间
DOI：
10.1111/tpj.14807
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Chun-Hai Dong
中科院分区：
文献类型：
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作者：
Yanchong Yu;Longfei Qiao;Jiacai Chen;Yongheng Rong;Yuhang Zhao;Xiankui Cui;Jinpeng Xu;Xiaomin Hou;Chun-Hai Dong
肌动蛋白解聚因子（ADF）是肌动蛋白细胞骨架动态组织的关键调节剂。有趣的是，人们发现ADF1基因沉默会延迟开花，但其机制仍不清楚。在这项研究中，ADF1被用作诱饵，通过酵母双杂交（Y2H）系统筛选其相互作用蛋白。其中之一是 REM16 转录因子。作为AP2/B3样转录因子家族成员之一，REM16含有两个B3结构域，其转录水平在花转变阶段不断增加。 REM16 的过度表达会加速开花，而 REM16 的沉默会延迟开花。基因表达分析表明，REM16过表达株系中关键开花激活基因CONSTANS(CO)、FLOWERING LOCUS T(FT)、LEAFY(LFY)和SUPPRESSOR OF OVEREXPRESSION OF CONSTANS(SOC1)表达上调，而开花抑制基因FLOWERING LOCUS C(FLC)转录下调。相反，与野生型植物相比，REM16基因沉默株系的CO、FT、LFY和SOC1的转录水平较低，但FLC的转录水平较高。体内外实验证明REM16可以直接结合SOC1和FT的启动子区。遗传分析支持 REM16 在开花途径中作用于 SOC1 和 FT 的上游。所有这些研究都提供了有力的证据证明REM16通过直接激活SOC1和FT来促进开花。
Actin depolymerizing factor (ADF) is a key modulator for dynamic organization of actin cytoskeleton. Interestingly, it was found that theADF1gene silencing delays flowering, but its mechanism remains unclear. In this study, ADF1 was used as a bait to screen its interacting proteins by the yeast two‐hybrid (Y2H) system. One of them, the REM16 transcription factor was identified. As one of the AP2/B3‐like transcriptional factor family members, the REM16 contains two B3 domains and its transcript levels kept increasing during the floral transition stage. Overexpression ofREM16accelerates flowering while silencing ofREM16delays flowering. Gene expression analysis indicated that the key flowering activation genes such asCONSTANS(CO),FLOWERING LOCUS T(FT),LEAFY(LFY) andSUPPRESSOR OF OVEREXPRESSION OF CONSTANS(SOC1) were upregulated in theREM16overexpression lines, while the transcription of the flowering suppression geneFLOWERING LOCUS C(FLC) was decreased. In contrast, theREM16gene silencing lines contained lower transcript levels of theCO,FT,LFYandSOC1but higher transcript levels of theFLCcompared with the wild‐type plants. It was proved that REM16 could directly bind to the promoter regions ofSOC1andFTbyin vitroandin vivoassays. Genetic analysis supported thatREM16acts upstream ofSOC1andFTin flowering pathways. All these studies provided strong evidence demonstrating that REM16 promotes flowering by directly activatingSOC1andFT.