Acetylcholine promotes the synthesis of prostaglandin E in mouse pancreas.
Acetylcholine promotes the synthesis of prostaglandin E in mouse pancreas.
复制标题
乙酰胆碱促进小鼠胰腺中前列腺素E的合成。
DOI：
10.1016/0014-5793(80)80928-8
复制
发表时间：
1980
期刊：
影响因子：
3.5
通讯作者：
Hokin-Neaverson,M
中科院分区：
文献类型：
--
作者：
Banschbach,MW;Hokin-Neaverson,M
主要的前列腺素（E2、FZa）是由花生酸合成的，花生酸可以通过磷脂或三酰基甘油的脱酰化而衍生[1]。考虑到用于前列腺素合成的花生四烯酸的精确来源，与其他磷脂相比，哺乳动物磷脂酰肌醇含有极高比例的花生四烯酸这一事实引起了人们的广泛关注。在小鼠胰腺中，总磷脂酰肌醇的 80% 是 1-硬脂酰、2-花生四烯酰。甘油磷酸肌醇种类[2]。乙酰胆碱 (ACh) 促进磷脂酰胆碱的净分解 [2, 3]，因此，这种分解可能会释放一些花生四烯酸，而花生四烯酸可以作为前列腺素合成的底物。然而，乙酰胆碱对前列腺素合成的影响尚未有报道。在这里，在已知促进该组织中磷脂酰肌醇分解的条件下检查了ACh对小鼠胰腺中前列腺素E（PGE）合成的影响。由于 PGEz 是大多数组织中由花生四烯酸合成的主要前列腺素，因此对 PGE 部分进行了测定 [11。
The major prostaglandins (E2, FZa) are synthesized from arac~ do~ c acid, which can be derived by deacylation of phospholipids or t~ acyl~ ycerols [l]. In considerations of the precise source of arachidonic acid used for prostaglandin synthesis, much attention has been drawn to the fact that mammalian phosphatidylinositol contains an exceptionally high proportion of arac~ do~ c acid, as compared with other phospholipids. In mouse pancreas,-80% of the total phosphatidyhnositol is the 1-stearoyl, 2-arachidonoylsn. glycerophosphorylinositol species [2]. Acetylcholine (ACh) promotes a net breakdown of this phosphatidyi~ o~ tol [2, 3] and it seemed possible, therefore, that there might be some arachidonic acid release associated with this breakdown which could act as a substrate for prostaglandin synthesis. An effect of ACh on prostaglandin synthesis has not been reported, however. Here, the effect of ACh on the synthesis of prost~~ d~ E (PGE) in mouse pancreas was examined under conditions which are known to promote the breakdown of phosphatidylinositol in this tissue. The PGE fraction was assayed since PGEz is the major prostaglandin synthesized from arachidonic acid in most tissues [11.