Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures
Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures
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DOI：
10.1007/s13361-015-1324-6
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发表时间：
2016-03-01
影响因子：
3.2
通讯作者：
Pasa-Tolic, Ljiljana
中科院分区：
文献类型：
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作者：
Anderton, Christopher R.;Chu, Rosalie K.;Pasa-Tolic, Ljiljana
使用MALDI MSI可视化微生物群落之间的生物化学相互作用的能力为各种生物学领域提供了巨大的见解。使用筛子的矩阵应用被证明是非常有用的，但它有许多局限性，包括不均匀的矩阵覆盖范围和可用于研究的矩阵类型的限制。最近，人们一直在努力改进用于研究琼脂平板微生物培养物的基质应用，其中许多使用自动基质喷雾器。在这里，我们描述了使用机器人喷雾器矩阵应用的有用性。机器人喷雾器具有对基质施加位置的二维控制，以及允许快速干燥所施加基质的加热毛细管。正如FT-ICR MS分析所证明的那样，与筛分方法相比，该方法显着提高了MALDI灵敏度，有助于获得比之前报道的更高横向分辨率的枯草杆菌MS图像。该方法还允许使用不同的基质应用于培养表面。
The ability to visualize biochemical interactions between microbial communities using MALDI MSI has provided tremendous insights into a variety of biological fields. Matrix application using a sieve proved to be incredibly useful, but it has many limitations that include uneven matrix coverage and limitation in the types of matrices that could be employed in studies. Recently, there has been a concerted effort to improve matrix application for studying agar plated microbial cultures, many of which utilized automated matrix sprayers. Here, we describe the usefulness of using a robotic sprayer for matrix application. The robotic sprayer has two-dimensional control over where matrix is applied, and a heated capillary that allows for rapid drying of the applied matrix. This method provided a significant increase in MALDI sensitivity over the sieve method, as demonstrated by FT-ICR MS analysis, facilitating the ability to gain higher lateral resolution MS images of Bacillus subtilis than previously reported. This method also allowed for the use of different matrices to be applied to the culture surfaces.