T cell responses in depressed mice induced by chronic unpredictable mild stress.
T cell responses in depressed mice induced by chronic unpredictable mild stress.
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DOI：
10.1016/j.jad.2021.09.064
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发表时间：
2021-09
影响因子：
6.6
通讯作者：
Wei Shi;Shan Zhang;Yujia Lu;Yaning Wang;Jing-jie Zhao;Li Li-Li
中科院分区：
文献类型：
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作者：
Wei Shi;Shan Zhang;Yujia Lu;Yaning Wang;Jing-jie Zhao;Li Li-Li
背景抑郁症与获得性免疫的关系逐渐受到关注。然而，没有一致的结论已经得出了关于适应性免疫在depression.Methods8周龄体重匹配的雄性C57 BL/6小鼠的改变随机分为慢性不可预测的轻度应激（CHNS）模型组和对照组，每组12只小鼠。经过8周的CIMIS建模时间和抑郁行为测试，T细胞反应在细胞，细胞因子和细胞特异性转录因子水平进行了评估。淋巴细胞在脾脏中进行了流式细胞术检测;血清细胞因子进行了评估，通过酶联免疫吸附试验，细胞因子和细胞特异性转录因子在海马的表达水平进行了测定，通过定量PCR.ResultsB2S曝光诱导抑郁样行为，减少蔗糖偏好和较长的不动时间在尾部悬挂试验和强迫游泳试验。CD 4 + T细胞的比例低于对照组。结果表明，BRAS组小鼠脾脏中Th 17细胞数量减少，血清IL-17和海马维甲酸相关孤儿受体γt水平下调。抑郁症组小鼠的血清IL-1β和IL-6以及海马IL-6和IL-4水平升高。结论硫化氢暴露可诱导抑郁样行为和Th 17细胞数量减少。
BackgroundThe relationship between depression and adaptive immunity has gradually attracted increasing attentions. However, no consistent conclusions have been drawn about alterations in adaptive immunity in depression.MethodsEight-week-old weight-matched male C57BL/6 mice were randomly divided into a chronic unpredictable mild stress (CUMS) model group and a control group, with twelve mice in each group. After 8-week CUMS modeling duration and depressive behavioral tests, T cell responses were evaluated at the cell, cytokine, and cell-specific transcription factor levels. Lymphocytes in the spleen were detected by flow cytometry; serum cytokines were assessed by enzyme-linked immunosorbent assays; expression levels of cytokines and cell-specific transcription factors in the hippocampus were determined by quantitative PCR.ResultsCUMS exposure induced depression-like behaviors with decreased sucrose preference and longer immobility time in the tail suspension test and forced swim test. The CUMS group had a lower proportion of CD4+Tcells than the control group. A decreased number of T helper 17 (Th17) cells in the spleen and down regulated serum interleukin (IL)-17 and hippocampal retinoid-related orphan receptor-γt levels were detected in the CUMS group. Depressed mice in the CUMS group showed increases in serum IL-1β and IL-6 and hippocampal IL-6 and IL-4 levels.LimitationThe study was based on a mouse model. Further studies are needed to determine whether the results are applicable to patients with depression.ConclusionCUMS exposure can induce depression-like behaviors and decrease Th17 cell counts.