Anastomosis groups, pathogenicity, and specificity of Rhizoctonia solani isolates from seedling and adult rapeseed/canola plants and soils in Saskatchewan
Anastomosis groups, pathogenicity, and specificity of Rhizoctonia solani isolates from seedling and adult rapeseed/canola plants and soils in Saskatchewan
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萨斯喀彻温省幼苗和成年油菜/双低油菜植物和土壤中分离的立枯丝核菌的吻合群、致病性和特异性
DOI：
10.1080/07060668709501904
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
R. Morrall
中科院分区：
文献类型：
--
作者：
S. Yitbarek;P. Verma;R. Morrall
材料和方法从患病植物中分离真菌。1984年6月27日至7月4日（幼苗）和7月27日至8月2日（成年植株）从萨斯喀彻温省中部主要油菜种植区的70个田地中收集了具有根腐样症状的油菜植株。将具有明显病变的根和基部茎部分切成片，在流动的自来水中冲洗以除去土壤，用10%Javex（0.6%NaOCl）表面灭菌（1分钟幼苗和2分钟成年植物），并在补充有0.001%硫酸链霉素的马铃薯葡萄糖琼脂（PDA）上铺板（每个培养皿4片）。使用来自每个样品的2至30个根/茎片。将平板在20 ℃下孵育，每24小时检查一次，持续一周.记录了不同真菌属的发生频率，但未尝试鉴定物种。
Materials and methods Sources and isolation of fungi from diseased plants. Canola plants with root rot-like symptoms were collected in 1984 between 27 June and 4 July (seedlings) and between 27 July and 2 August (adult plants) from 70 fields in the main canola growing areas of central Saskatchewan. Roots and basal stem portions with distinct lesions were cut in pieces, rinsed in running tap water to remove soil, surface sterilized with 10% Javex (0.6% NaOCl)(1 min seedlings and 2 min adult plants), and plated (4 pieces per petri dish) on potato dextrose agar (PDA) supplemented with 0.001% streptomycin sulphate. Two to 30 root/stem pieces from each sample were used. Plates were incubated at 20 C and examined every 24 h for one week. The frequency of occurrence of different fungal genera was recorded but no attempt was made to identify species.