Intraskeletal isotopic compositions (δ(13) C, δ(15) N) of bone collagen: nonpathological and pathological variation.
Intraskeletal isotopic compositions (δ(13) C, δ(15) N) of bone collagen: nonpathological and pathological variation.
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骨胶原的骨骼内同位素组成（δ(13) C、δ(15) N）：非病理性和病理性变化。
DOI：
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发表时间：
2014
影响因子：
2.8
通讯作者：
F. Rühli
中科院分区：
文献类型：
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作者：
Karyn C. Olsen;C. White;F. Longstaffe;Kristina von Heyking;George C. McGlynn;G. Grupe;F. Rühli
古饮食研究传统上将胶原蛋白同位素组成（δ（13）C，δ（15）N）的差异解释为饮食差异的指标，尽管生理过程可能在产生差异方面发挥一定作用。这项研究调查了骨胶原δ（13）C和δ（15）N值在骨骼内正常变化的程度，并检查了古代人群常见的几种疾病对这些同位素组成的影响。这些样本来自两个中世纪德国墓地和一个瑞士参考收藏品，包括代谢性疾病（佝偻病/骨软化症）、退行性关节疾病（骨关节炎）、创伤（骨折）、感染（骨髓炎）和炎症（骨膜炎）。一个单独的子集，明显的非病理性骨骼元素从德国收集建立正常的个体内变异。对于每种疾病类型，测试将骨病变样本与病变部位附近和远处的样本进行比较。结果表明，正常（非病理性）的骨骼表现出有限的碳和氮同位素比值的intrasketum变化，这表明不同元素的采样是适当的古饮食研究。与此相反，骨髓炎、骨折愈合和骨关节炎患者的同位素值在骨内表现出显著差异，这取决于是否将病变与近部位或远部位进行比较。患有骨膜炎的骨质疏松症仅导致氮同位素值的显著骨内差异，而患有佝偻病/骨软化症的骨质疏松症患者则没有表现出显著的骨内差异。基于这些结果，我们建议古饮食研究人员避免在病变部位或病变部位附近采集胶原蛋白，因为同位素值可能反映了代谢过程的改变和饮食相对于人群中其他人的差异。
Paleodiet research traditionally interprets differences in collagen isotopic compositions (δ(13) C, δ(15) N) as indicators of dietary distinction even though physiological processes likely play some role in creating variation. This research investigates the degree to which bone collagen δ(13) C and δ(15) N values normally vary within the skeleton and examines the influence of several diseases common to ancient populations on these isotopic compositions. The samples derive from two medieval German cemeteries and one Swiss reference collection and include examples of metabolic disease (rickets/osteomalacia), degenerative joint disease (osteoarthritis), trauma (fracture), infection (osteomyelitis), and inflammation (periostitis). A separate subset of visibly nonpathological skeletal elements from the German collections established normal intraindividual variation. For each disease type, tests compared bone lesion samples to those near and distant to the lesions sites. Results show that normal (nonpathological) skeletons exhibit limited intraskeletal variation in carbon- and nitrogen-isotope ratios, suggesting that sampling of distinct elements is appropriate for paleodiet studies. In contrast, individuals with osteomyelitis, healed fractures, and osteoarthritis exhibit significant intraskeletal differences in isotope values, depending on whether one is comparing lesions to near or to distant sites. Skeletons with periostitis result in significant intraskeletal differences in nitrogen isotope values only, while those with rickets/osteomalacia do not exhibit significant intraskeletal differences. Based on these results, we suggest that paleodiet researchers avoid sampling collagen at or close to lesion sites because the isotope values may be reflecting both altered metabolic processes and differences in diet relative to others in the population.