Local protein synthesis by BDNF is potentiated in hippocampal neurons exposed to ephrins
Local protein synthesis by BDNF is potentiated in hippocampal neurons exposed to ephrins
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DOI：
10.1016/j.molbrainres.2004.10.034
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发表时间：
2005-04-04
期刊：
影响因子：
--
通讯作者：
Tohyama, M
中科院分区：
文献类型：
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作者：
Miyata, S;Mori, Y;Tohyama, M
神经元树突中的局部蛋白质合成是可能介导来自驻留 mRNA 的蛋白质快速和突触特异性动员的机制之一。人们在主要通过定量蛋白质水平来分析活细胞中蛋白质合成的动态方面做出了大量的努力。然而，蛋白质水平无法反映翻译的时空变化，因为它不仅会受到蛋白质合成的影响，还会受到其他因素（例如降解）的影响。因此，通过现有的方法来可视化翻译的动态状态是有问题的。为了解决这个问题，我们应用荧光共振能量转移（FRET）技术原位检测一对翻译起始因子[真核起始因子（elFs）]之间的组装和解组装循环，从而表明BDNF和肝配蛋白可以增强海马神经元树突中的局部蛋白质合成。 (c) 2004 Elsevier B.V 保留所有权利。
Localprotein synthesis in neuronal dendrites is one of the mechanisms that may mediate a rapid and synapse-specific mobilization of proteins from the resident mRNAs. A great deal of effort has been made in analyzing the dynamic state of protein synthesis in the living cells chiefly by quantifying protein level. However, the protein level cannot mirror the spatiotemporal alteration of translation because it can be affected, not only by protein synthesis, but also by other factors, like degradation. Therefore, it is problematic to visualize the dynamic state of translation by the present methods'. To solve the problem, we applied fluorescence resonance energy transfer (FRET) technique to in situ detection of the assembly and disassembly cycle among a pair of translation initiation factors [eukaryotic initiation factors (elFs)], thereby showing that BDNF and ephrin could potentiate local protein synthesis in the dendrites of hippocampal neurons. (c) 2004 Elsevier B.V All rights reserved.