Investigating virus-glycan interactions using glycan microarrays
Investigating virus-glycan interactions using glycan microarrays
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DOI：
10.1016/j.coviro.2014.05.005
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发表时间：
2014-08-01
影响因子：
5.9
通讯作者：
Cummings, Richard D.
中科院分区：
文献类型：
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作者：
Smith, David F.;Cummings, Richard D.
虽然所有病毒都必须通过哺乳动物细胞的质膜来启动感染，但我们对病毒附着中涉及的复杂过程知之甚少，这些过程通常涉及病毒蛋白对聚糖的识别。从合成聚糖和通过鸟枪糖组学方法分离的天然聚糖衍生的聚糖微阵列为询问作为潜在病毒受体的不同聚糖提供了新的平台。最近使用这种聚糖微阵列对流感病毒和轮状病毒的研究提供了它们在探索挑战性问题中的效用的例子，这些挑战性问题是在努力定义调节病毒附着到宿主细胞的复杂机制蛋白质-聚糖相互作用中提出的。
While all viruses must transit the plasma membrane of mammalian cells to initiate infection, we know little about the complex processes involved in viral attachment, which commonly involve recognition of glycans by viral proteins. Glycan microarrays derived from both synthetic glycans and natural glycans isolated through shotgun glycomics approaches provide novel platforms for interrogating diverse glycans as potential viral receptors. Recent studies with influenza and rotaviruses using such glycan microarrays provide examples of their utility in exploring the challenging questions raised in efforts to define the complex mechanistic protein-glycan interactions that regulate virus attachment to host cells.