Cucumber mosaic virus Isolates from Greek Legumes are Associated with Satellite RNAs that are Necrogenic for Tomato
Cucumber mosaic virus Isolates from Greek Legumes are Associated with Satellite RNAs that are Necrogenic for Tomato
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DOI：
10.1094/pdis-08-17-1259-re
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发表时间：
2018-11-01
期刊：
影响因子：
4.5
通讯作者：
Chatzivassiliou, Elisavet K.
中科院分区：
文献类型：
--
作者：
Giakountis, Antonis;Tsarmpopoulos, Iason;Chatzivassiliou, Elisavet K.
黄瓜花叶病毒（CMV）是世界范围内许多重要病害的病原。基于免疫学或分子分析，可以鉴定CMV分离株的三个不同亚组（IA、IB和II）。此外，一些CMV分离株与卫星RNA（satRNA）相关，卫星RNA是一种非编码转录物，可能会改变CMV感染的症状。本研究提出了一种分析CMV分离物发生在豆类在希腊方面的遗传多样性，其卫星RNA的存在和多样性。系统发育分析的CMV外壳蛋白序列的18个豆类和5个番茄CMV分离物收集在整个希腊的亚组IA和IB，有限的遗传多样性内分类。CMV-satRNA发现在9个领域豆科植物表现出轻微的症状，并在一个番茄坏死综合征包含一个功能性坏死基序，因此，它们被分组在CMV-satRNA的坏死组。当机械接种到番茄植株上时，在所有含有致坏死卫星RNA的豆科CMV分离物中表达坏死表型。据我们所知，这是第一次观察到豆类宿主坏死CMV-satRNA。豆科植物在CMV和坏死性卫星RNA复合物的流行病学中可能发挥的作用进行了讨论。
Worldwide, Cucumber mosaic virus (CMV) is the causal agent of many economically important diseases. Based on immunological or molecular analysis, three distinct subgroups of CMV isolates can be identified (IA, IB, and II). In addition, some CMV isolates are associated with satellite RNAs (satRNAs), a type of noncoding transcript that may alter the symptoms of CMV infections. This study presents an analysis of CMV isolates occurring in legumes in Greece in respect to their genetic diversity, and the presence and diversity of their satRNA. Phylogenetic analysis of the CMV coat protein sequence of 18 legume and 5 tomato CMV isolates collected throughout Greece classified them within subgroups IA and IB, with a limited genetic diversity. The CMV-satRNAs found in nine field legumes exhibiting mild symptoms and in one tomato with a necrotic syndrome contained a functional necrogenic motif; therefore, they were grouped within the necrogenic group of CMV-satRNAs. The necrotic phenotype was expressed in all legume CMV isolates containing necrogenic satRNAs when mechanically inoculated onto tomato plants. To our knowledge, this is the first observation that legumes host necrogenic CMV-satRNAs. The possible role of legumes in the epidemiology of CMV and necrogenic satRNA complex is discussed.