Stabilization of gelatin films by crosslinking with genipin
Stabilization of gelatin films by crosslinking with genipin
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DOI：
10.1016/s0142-9612(02)00235-1
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发表时间：
2002-12-01
期刊：
影响因子：
14
通讯作者：
Rubini, K
中科院分区：
文献类型：
--
作者：
Bigi, A;Cojazzi, G;Rubini, K
通过用不同浓度的京尼平溶液处理的样品的机械、化学和热表征，研究了通过与京尼平交联来稳定明胶膜的可能性。作为交联前后游离α-氨基数目之间的差异评估的交联程度作为京尼平浓度的函数增加至约85%。同时，薄膜的变形能力降低，而杨氏模量E1增加。此外，交联引起在生理溶液中的溶胀的显著降低，并且增强样品的热稳定性，如由d.s.c.调查从用浓度高于0.67%的京尼平处理的膜获得的数据非常相似，并且表明京尼平的良好稳定效果。尽管在缓冲溶液中储存1个月后观察到少量明胶释放（2%），但薄膜的机械、热和溶胀性能非常接近先前获得的戊二醛交联明胶的性能，并表明细胞毒性小得多的京尼平可被视为交联明胶生物材料的有效替代品。(C)2002爱思唯尔科技有限公司版权所有。
The possibility to stabilize gelatin films by crosslinking with genipin was investigated through a mechanical, chemical and thermal characterization of samples treated with genipin solutions at different concentrations. The extent of crosslinking, evaluated as difference between the number of free a-amino groups before and after crosslinking, increases as a function of genipin concentration up to about 85%. Simultaneously, the deformability of the films decreases whereas the Young's modulus E, increases. Furthermore, crosslinking provokes a significant reduction of the swelling in physiological solution, and enhances the thermal stability of the samples, as indicated by the results of the d.s.c. investigation. The data obtained from the films treated with genipin at concentrations higher than 0.67% are quite similar, and indicative of a good stabilizing effect of genipin. In spite of the small gelatin release (2%) observed after I month of storage in buffer solution, the mechanical, thermal and swelling properties of the films are very close to those previously obtained for glutaraldehyde crosslinked gelatin, and suggest that genipin, which is by far less cytotoxic, can be considered a valid alternative for crosslinking gelatin biomaterials. (C) 2002 Elsevier Science Ltd. All rights reserved.