Composite system based on chitosan and room-temperature ionic liquid: Direct electrochemistry and electrocatalysis of hemoglobin
Composite system based on chitosan and room-temperature ionic liquid: Direct electrochemistry and electrocatalysis of hemoglobin
复制标题
DOI：
10.1021/bm050933t
复制
发表时间：
2006-03-01
影响因子：
6.2
通讯作者：
Li, JH
中科院分区：
文献类型：
--
作者：
Lu, XB;Hu, JQ;Li, JH
以壳聚糖（Chi）和1-丁基-3-甲基咪唑四氟硼酸盐（BMIMBF 4）为原料，制备了一种新型的聚合物/室温离子液体（RTIL）复合材料。该复合体系可以很容易地用作包埋蛋白质和酶的固定化基质。以血红蛋白（Hb）为模型蛋白，研究了复合体系。在Chi-BMIMBF 4-Hb复合膜修饰玻碳电极上，血红蛋白通过直接电子转移在玻碳电极上产生一对准可逆的氧化还原峰。通过氧和三氯乙酸的还原，在Chi-BMIMBF 4-Rb/GC电极处例示了显著增强的生物催化活性。热重分析（TGA）表明，Chi-BMIMBF 4-Hb复合物具有比Chi-Hb本身更高的热稳定性。Chi-BMIMBF 4-Hb膜的紫外-可见光谱也进行了表征，表明在溶液中具有优异的稳定性和良好的蛋白质生物相容性。聚合物与离子液体的独特复合材料在直接电化学、生物传感器和生物催化等领域具有广阔的应用前景。
A novel polymer/room-temperature ionic liquid (RTIL) composite material based on chitosan (Chi) and 1-butyl-3-methyl-imidazolium tetrafluoroborate (BMIMBF4) was explored. The composite system can be readily used as an immobilization matrix to entrap proteins and enzymes. Hemoglobin (Hb) was chosen as a model protein to investigate the composite system. A pair of well-defined quasireversible redox peaks of hemoglobin were obtained at the Chi-BMIMBF4-Hb composite-film-modified glassy carbon (GC) electrode by direct electron transfer between the protein and the GC electrode. Dramatically enhanced biocatalytic activity was exemplified at the Chi-BMIMBF4-Rb/GC electrode by the reduction of oxygen and trichloroacetic acid. Thermogravimetric analysis (TGA) suggests that the Chi-BMIMBF4-Hb composite has higher thermal stability than Chi-Hb itself. The Chi-BMIMBF4-Hb film was also characterized by UV-visible spectra, indicating excellent stability in solution and good biocompatibility for protein. The unique composite material based on polymer and ionic liquid can find wide potential applications in direct electrochemistry, biosensors, and biocatalysis.