The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs
The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs
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三磷酸肌醇和钙在海胆卵受精波传播中的作用
作者：
K. Swann;M. Whitaker
受精时海胆卵的激活是渐进的，从精子进入点开始，以5微米/秒的速度穿过卵。这种激活波（Kacser，H.，1955，J. Exp.生物学：32：451-467）已经被认为是钙从卵细胞质内的储存中进行性释放的结果（Jaffe，L. F.、1983年，德夫。生物学：99：265-276）。钙的逐步释放可能是由于第二信使三磷酸肌醇（InsP 3）的产生。我们在这里表明，钙释放的波穿过Lytechinus pictus卵;波的峰值以5微米/秒的速度传播;微量注射InsP 3导致卵内钙的释放;钙释放（通过受精包膜升高判断）被预先注射钙螯合剂EGTA消除;新霉素是InsP 3产生的抑制剂，不阻止响应InsP 3的钙释放，但确实消除了钙释放波;卵细胞质迅速缓冲显微注射的钙;当显微注射时引起受精膜升高所需的钙浓度与体外刺激InsP 3产生所需的钙浓度非常相似;显微注射钙缓冲液后受精膜的逐渐升高似乎是由于缓冲液在卵中的扩散细胞质，而不是激活波的诱导。我们的结论是，InsP 3扩散通过卵细胞质更容易比钙离子和钙刺激生产InsP 3和InsP 3诱导的钙释放从内部存储可以解释在受精的钙的逐步释放。
Sea urchin egg activation at fertilization is progressive, beginning at the point of sperm entry and moving across the egg with a velocity of 5 microns/s. This activation wave (Kacser, H., 1955, J. Exp. Biol., 32:451-467) has been suggested to be the result of a progressive release of calcium from a store within the egg cytoplasm (Jaffe, L. F., 1983, Dev. Biol., 99:265-276). The progressive release of calcium may be due to the production of inositol trisphosphate (InsP3), a second messenger. We show here that a wave of calcium release crosses the Lytechinus pictus egg; the peak of the wave travels with a velocity of 5 microns/s; microinjection of InsP3 causes the release of calcium within the egg; calcium release (as judged by fertilization envelope elevation) is abolished by prior injection of the calcium chelator EGTA; neomycin, an inhibitor of InsP3 production, does not prevent the release of calcium in response to InsP3 but does abolish the wave of calcium release; the egg cytoplasm rapidly buffers microinjected calcium; the calcium concentration required to cause fertilization membrane elevation when microinjected is very similar to that required to stimulate the production of InsP3 in vitro; and the progressive fertilization membrane elevation seen after microinjection of calcium buffers appears to be due to diffusion of the buffer across the egg cytoplasm rather than to the induction of the activation wave. We conclude that InsP3 diffuses through the egg cytoplasm much more readily than calcium ions and that calcium-stimulated production of InsP3 and InsP3-induced calcium release from an internal store can account for the progressive release of calcium at fertilization.
三磷酸肌醇诱导海胆卵匀浆中非线粒体储存的钙释放。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Clapper,DL;Lee,HC
通讯作者：
Lee,HC
磷脂酶 C 裂解磷酸肌醇的肌醇环磷酸产物的分离和表征。无细胞提取物中的代谢。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Connolly,TM;Wilson,DB;Bross,TE;Majerus,PW
通讯作者：
Majerus,PW
磷脂酶 C 裂解多磷酸肌醇的肌醇环磷酸产物的分离和表征。透化血小板和鲎感光细胞的生理效应。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wilson,DB;Connolly,TM;Bross,TE;Majerus,PW;Sherman,WR;Tyler,AN;Rubin,LJ;Brown,JE
通讯作者：
Brown,JE