Transplantation of Adipose-Derived Stem Cell Sheet Attenuates Adverse Cardiac Remodeling in Acute Myocardial Infarction
Transplantation of Adipose-Derived Stem Cell Sheet Attenuates Adverse Cardiac Remodeling in Acute Myocardial Infarction
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DOI：
10.1089/ten.tea.2016.0023
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发表时间：
2017-01-01
影响因子：
4.1
通讯作者：
Lim, Do-Sun
中科院分区：
文献类型：
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作者：
Kim, Jong-Ho;Joo, Hyung Joon;Lim, Do-Sun
脂肪源性干细胞（ADSC）移植已被提出用于改善心功能和急性心肌梗死（AMI）。近年来，人们对细胞片技术在心脏损伤中的潜在应用进行了研究。然而，细胞片和常规细胞注射对损伤心肌功能恢复的详细比较尚未得到充分的研究。从ICR小鼠腹股沟脂肪组织中分离ADSCs。采用大鼠AMI模型，比较单纯AMI诱导（sham）、心内注射ADSC （imADSC）和ADSC片移植（shADSC）三组AMI的差异。与imADSC组相比，shADSC组的ADSCs在28天内的移植效果更好。与假手术组相比，imADSC组和shADSC组的射血分数均有改善。与imADSC组和sham组相比，shADSC组梗死区室壁厚度更高。与imADSC组和sham组相比，shADSC组移植心脏组织中生长因子和细胞因子的表达更高。此外，只有shADSC组在梗死周围区显示供体来源的血管。综上所述，这些结果表明，尽管与imADSC相比，shADSC对左心室收缩功能的改善类似，但它显著促进了细胞植入，上调了生长因子和细胞因子的表达，并最终减轻了AMI模型大鼠的不良心脏重构。
Adipose-derived stem cell (ADSC) transplantation has been proposed to improve cardiac function and acute myocardial infarction (AMI). Recently, cell sheet technology has been investigated for its potential applicability in cardiac injury. However, a detailed comparison of the functional recovery in the injured myocardium between cell sheets and conventional cell injection has not been adequately examined. ADSCs were isolated from the inguinal fat tissue of ICR mice. Three groups of AMI induction only (sham), intramyocardial injection of ADSCs (imADSC), and ADSC sheet transplantation (shADSC) were compared by using rat AMI models. Engraftment of ADSCs was better sustained through 28 days in the shADSC group compared with the imADSC group. Ejection fraction was improved in both imADSC and shADSC groups compared with the sham group. Ventricular wall thickness in the infarct zone was higher in the shADSC group compared with both imADSC and sham groups. Growth factor and cytokine expression in the implanted heart tissue were higher in the shADSC group compared with both imADSC and sham groups. Furthermore, only the shADSC group showed donor-derived vessels at the peri-infarct zone. Taken together, these results indicate that, although shADSC resulted in a similar improvement in left ventricular systolic function, it significantly promoted cellular engraftment and upregulated growth factor and cytokine expression, and, ultimately, attenuated adverse cardiac remodeling in rat AMI models compared with imADSC.