Gene characterization of sciellin (SCEL) and protein localization in vertebrate epithelia displaying barrier properties
Gene characterization of sciellin (SCEL) and protein localization in vertebrate epithelia displaying barrier properties
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DOI：
10.1006/geno.2000.6390
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发表时间：
2000-12-01
期刊：
影响因子：
4.4
通讯作者：
Viel, A
中科院分区：
文献类型：
--
作者：
Champliaud, MF;Baden, HP;Viel, A
鞘蛋白是哺乳动物层状上皮的锥形包膜的前体，其特征是中心核心的不相同重复。我们对人类sciellin的基因组结构进行了表征，发现每个同源重复序列由一个外显子编码。我们还对小鼠sciellin进行了表征，发现小鼠sciellin和人类sciellin （hgmw批准的符号SCEL）具有相似的基因组织和蛋白表达模式。这种一个外显子/一个重复的组织在其他以同源重复为特征的包膜前体中是独一无二的。Me鉴定了人类sciellin的一种选择性剪接异构体，在小鼠中不存在，其特征是在核心结构域的开始有一个额外的重复。胚胎发育过程中，鞘蛋白转录物和鞘蛋白在表皮的积累与表皮终末分化标志物的激活有关。小鼠鞘蛋白也在简单上皮中被发现具有屏障特性，进一步支持其在上皮功能中的重要性，(C) 2000学术出版社。
Sciellin is a precursor of the cornified envelope of mammalian stratified epithelia characterized by a central core of nonidentical repeats. We characterized the genomic structure of human sciellin and showed that each homologous repeat was encoded by one exon. We also characterized mouse sciellin and showed that mouse sciellin and human sciellin (HGMW-approved symbol SCEL) share a similar gene organization and protein expression pattern. This one exon/one repeat organization is unique among other cornified envelope precursors characterized by homologous repeats. me identified an alternatively spliced isoform of human sciellin, absent in mouse, characterized by an additional repeat at the beginning of the core domain. During embryonic development, the accumulation of sciellin transcript and the accumulation of sciellin protein in the epidermis correlated with the activation of markers of terminal differentiation in epidermis. Mouse sciellin was also identified in simple epithelia with barrier properties, lending further support to its importance in epithelial function, (C) 2000 Academic Press.