31P-NMR study of the activation of the sea urchin egg.
31P-NMR study of the activation of the sea urchin egg.
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海胆卵活化的 31P-NMR 研究。
DOI：
10.1016/0014-4827(82)90335-4
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发表时间：
1982
影响因子：
3.7
通讯作者：
Bradbury,EM
中科院分区：
文献类型：
--
作者：
Winkler,MM;Matson,GB;Hershey,JW;Bradbury,EM
31 P-NMR已被用作非侵入性探针，以检查海胆卵受精时pH和磷酸盐代谢的变化。施肥后测得pH值增加0.43个单位。该值与pH微电极和DMO方法的测量结果非常一致，但与其他工作人员未能用31 P-NMR测量pH值增加形成对比。显着增加精氨酸磷酸与受精被认为是不触发由NH 4C 1激活诱导的pH值增加。此外，我们还检测了海胆精子的~（31）P-NMR谱，并测定了它们的细胞内pH值。
31P-NMR has been used as a non-invasive probe to examine changes in pH and phosphate metabolism at fertilization of the sea urchin egg. A pH increase of 0.43 units was measured following fertilization. This value is in good agreement with measurements made with pH micro-electrodes and the DMO method, but is contrasted with the failure of other workers to measure a pH increase with31P-NMR. Significantly the increase in arginine phosphate associated with fertilization was found not to be triggered by a pH increase induced by NH4C1 activation. In addition we examined the31P-NMR spectrum of sea urchin sperm and determined their intracellular pH.