Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures.

Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures.
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颈上神经节外植体培养中的轴突变性测定。

DOI:
10.1007/978-1-0716-0585-1_2
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发表时间:
2020
影响因子:
--
通讯作者:
J. Gilley
J. Gilley
中科院分区:
--
文献类型:
--
作者:
Andrea Loreto;J. Gilley

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外周神经系统神经元在体外能够延伸出长的轴突样突起,这使得它们非常适合于轴突存活和退化机制的研究。在本章中,我们描述了如何制备颈上神经节(SCG)交感神经元的外植体培养。我们还描述了如何通过损伤或化学侮辱来诱导和评估轴突变性。
The ability of peripheral nervous system neurons to extend long, axon-like neurites in vitro makes them ideally suited for studies on mechanisms of axon survival and degeneration. In this chapter, we describe how to prepare explant cultures of sympathetic neurons of the superior cervical ganglion (SCG). We also describe how to induce and assess axon degeneration with an injury or a chemical insult.
DOI: 10.1016/j.celrep.2015.11.032
发表时间: 2015-12-22
期刊: CELL REPORTS
影响因子: 8.8
作者:
Loreto, Andrea;Di Stefano, Michele;Conforti, Laura
通讯作者: Conforti, Laura