Contact with basement membrane heparan sulfate enhances the growth of transformed vascular endothelial cells, but suppresses normal cells.
Contact with basement membrane heparan sulfate enhances the growth of transformed vascular endothelial cells, but suppresses normal cells.
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与基底膜硫酸乙酰肝素接触可增强转化血管内皮细胞的生长，但抑制正常细胞。
DOI：
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发表时间：
1991
影响因子：
1.5
通讯作者：
Y. Mitsui
中科院分区：
文献类型：
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作者：
T. Imamura;Y. Tokita;Y. Mitsui
用四种正常和转化细胞系研究了基底膜硫酸乙酰肝素对血管内皮细胞生长的调节作用。当膜的成分之一硫酸乙酰肝素被一种酶（肝素酶）特异性降解时，转化的内皮细胞（而不是正常细胞）在重建的基底膜上的生长受到严重抑制。类似地，当细胞在用硫酸乙酰肝素包被的表面上生长时，低至60 pg/cm 2的硫酸乙酰肝素引起转化细胞的生长增强，但抑制正常细胞。这些结果与我们以前的观察（IMAMURA，T和MITSUI，Y。（1987）Exp. Cell Res.，一百七十二：92-100）认为转化细胞已经逆转了基底膜硫酸乙酰肝素作为正常内皮细胞生长的生理抑制剂的机制。
Modulation of vascular endothelial cell growth by basement membrane heparan sulfate was investigated using four lines of normal and transformed cells. The growth of transformed endothelial cells, but not normal cells, on reconstituted basement membrane was severely suppressed when heparan sulfate, one of the components of the membrane, was specifically degraded by an enzyme, heparitinase. Similarly, when cells were grown on surfaces coated with heparan sulfate, as little as 60 pg/cm2 of heparan sulfate caused growth enhancement of transformed cells, but suppression of normal cells. These results together with our previous observations (IMAMURA, T and MITSUI, Y. (1987) Exp. Cell Res., 172: 92-100) argue that transformed cells have reversed a mechanism by which basement membrane heparan sulfate functions as a physiological suppressor for the growth of normal endothelial cells.
多种形式的内皮细胞生长因子。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Burgess,WH;Mehlman,T;Friesel,R;Johnson,WV;Maciag,T
通讯作者：
Maciag,T