Photo‐Powered Artificial Organelles for ATP Generation and Life‐Sustainment
Photo‐Powered Artificial Organelles for ATP Generation and Life‐Sustainment
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DOI：
10.1002/adma.201805038
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发表时间：
2018-10
影响因子：
29.4
通讯作者：
Diwei Zheng;Lu Xu;Chuxin Li;Xue Dong;Pei Pan;Qiu-Ling Zhang;Bin Li;Xuan Zeng;Xianzheng Zhang
中科院分区：
文献类型：
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作者：
Diwei Zheng;Lu Xu;Chuxin Li;Xue Dong;Pei Pan;Qiu-Ling Zhang;Bin Li;Xuan Zeng;Xianzheng Zhang
三磷酸腺苷（ATP）是几乎所有生命形式中驱动细胞内生化反应的最重要的直接能量来源。在生活中可控地产生ATP仍然是一个未实现的目标。在这里，类囊体片段与脂质分子重组以合成合成/生物混合蛋白脂质体，命名为用于产生ATP的高效生命支持细胞内光驱动系统（HELIOS）。在红光照射下，HELIOS可使各种细胞系的细胞内ATP浓度提高1.38-2.45倍。此外，值得注意的是，HELIOS介导的ATP生成可以全面促进细胞功能，如蛋白质合成和胰岛素分泌。在器官和个体水平，也证明了HELIOS可以挽救心肌梗死的小鼠心脏和维持禁食斑马鱼模型的生命。光动力人工细胞器可以加深我们对新陈代谢的理解，并使针对细胞内能量供应的光学治疗得以发展。
Adenosine triphosphate (ATP) is the most important immediate energy source for driving intracellular biochemical reactions in nearly all life forms. Controllable generation of ATP in life is still an unrealized goal. Here, thylakoid fragments are recombined with lipid molecules to synthesize a synthetic/biological hybrid proteoliposome, named highly efficient life‐support intracellular opto‐driven system (HELIOS) for the generation of ATP. With red light irradiation, HELIOS can improve the intracellular ATP concentration to 1.38–2.45 times in various cell lines. Moreover, it is noticed that HELIOS‐mediated ATP generation can comprehensively promote cell functions such as protein synthesis and insulin secretion. At organ and individual levels, it is also proved that HELIOS can rescue a mouse heart from myocardial infarction and sustain life of fasting zebrafish Danio rerio models. The photo‐powered artificial organelle can deepen our understanding of metabolism and enable the development of optical therapy that targets intracellular energy supply.