PAX3 inhibits β-Tubulin-III expression and neuronal differentiation of neural stem cell
PAX3 inhibits β-Tubulin-III expression and neuronal differentiation of neural stem cell
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PAX3抑制β-Tubulin-III表达和神经干细胞的神经元分化
DOI：
10.1016/j.bbrc.2017.02.086
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发表时间：
2017-04-01
影响因子：
3.1
通讯作者：
Liu, Yan
中科院分区：
文献类型：
--
作者：
Cao, Sixian;Du, Jinfeng;Liu, Yan
PAX3在神经干细胞的维持和分化中起着节点作用。利用生物信息学方法，我们发现PAX3是β -微管蛋白- iii （TUBB3）基因转录的潜在调节因子，结果表明PAX3可能通过协调细胞骨架蛋白的表达参与神经干细胞（NSC）的分化。在本研究中，我们报道了PAX3可以抑制NSCs的分化和TUBB3的表达。此外，通过荧光素酶和电泳迁移转移实验，我们证明PAX3可以结合到TUBB3的启动子区域并抑制TUBB3的转录。最后，我们证实了PAX3可以结合NSCs天然染色质中内源性TUBB3的启动子区域。这些发现表明PAX3是NSCs体外分化过程中靶向多种分子的关键因子。(C) 2017爱思唯尔公司版权所有。
PAX3 functions at the nodal point in neural stem cell maintenance and differentiation. Using bioinformatics methods, we identified PAX3 as a potential regulator of beta-Tubulin-III (TUBB3) gene transcription, and the results indicated that PAX3 might be involved in neural stem cell (NSC) differentiation by orchestrating the expression of cytoskeletal proteins. In the present study, we reported that PAX3 could inhibit the differentiation of NSCs and the expression of TUBB3. Further, using luciferase and electrophoretic mobility shift assays, we demonstrated that PAX3 could bind to the promoter region of TUBB3 and inhibit TUBB3 transcription. Finally, we confirmed that PAX3 could bind to the promoter region of endogenous TUBB3 in the native chromatin of NSCs. These findings indicated that PAX3 is a pivotal factor targeting various molecules during differentiation of NSCs in vitro. (C) 2017 Elsevier Inc. All rights reserved.