A modular, dynamic, DNA-based platform for regulating cargo distribution and transport between lipid domains
A modular, dynamic, DNA-based platform for regulating cargo distribution and transport between lipid domains
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一个模块化、动态、基于 DNA 的平台，用于调节脂质域之间的货物分配和运输
DOI：
10.1101/2021.03.02.433457
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Rubio-Sánchez R
中科院分区：
文献类型：
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作者：
Rubio-Sánchez R
细胞膜调节生物机械在不同相分离的脂域之间的分布，以促进包括信号和运输在内的关键过程，这些是自下而上的合成生物学旨在在人工细胞系统中复制的类生命功能之一。在这里，我们介绍了一种模块化的方法来对共存类脂结构域中的两亲性DNA纳米结构进行程序划分。利用不同的疏水“锚”来丰富不同的相的趋势，我们通过合理地结合疏水分子和改变纳米结构的尺寸和拓扑结构来调节器件的横向分布。我们用一个生物启发的DNA架构展示了我们策略的功能，它动态地经历配体诱导的重新配置，通过横向重新分布在结构域之间调解货物运输。我们的发现为下一代仿生平台在合成细胞系统中进行传感、转导和通信铺平了道路。
Cell membranes regulate the distribution of biological machinery between phase-separated lipid domains to facilitate key processes including signaling and transport, which are among the life-like functionalities that bottom-up synthetic biology aims to replicate in artificial-cellular systems. Here, we introduce a modular approach to program partitioning of amphiphilic DNA nanostructures in coexisting lipid domains. Exploiting the tendency of different hydrophobic “anchors” to enrich different phases, we modulate the lateral distribution of our devices by rationally combining hydrophobes and by changing nanostructure size and topology. We demonstrate the functionality of our strategy with a bioinspired DNA architecture, which dynamically undergoes ligand-induced reconfiguration to mediate cargo transport between domains via lateral redistribution. Our findings pave the way to next-generation biomimetic platforms for sensing, transduction, and communication in synthetic cellular systems.