Mechanism of bradykinin-induced cyclic GMP accumulation in bovine tracheal smooth muscle.
Mechanism of bradykinin-induced cyclic GMP accumulation in bovine tracheal smooth muscle.
复制标题
缓激肽诱导牛气管平滑肌环鸟苷酸积累的机制。
作者：
Sheng,H;Ishii,K;Förstermann,U;Murad,F
在没有上皮的情况下，缓激肽 (10−8-10−5M) 会导致牛气管平滑肌中环 GMP (cGMP) 产生浓度依赖性增加。该作用是钙依赖性的，并被连苯三酚 (10 μM) 和亚甲蓝 (10 μM) 抑制。超氧化物歧化酶（100 Usnowml）可逆转邻苯三酚的抑制作用。一氧化氮（NO）合酶抑制剂NG-甲基-L-精氨酸（10-100 μM）和NG-硝基-L-精氨酸（10-100 μM）以浓度依赖性方式减少缓激肽诱导的cGMP积累，并且L-精氨酸可逆转这种抑制作用。使用针对大鼠小脑神经元 NO 合酶的特异性抗体进行的免疫组织化学显示，局部神经纤维呈阳性染色。缓激肽诱导的 cGMP 积累似乎与 NO 的释放有关，其中一部分可能是在牛气管的非肾上腺素能非胆碱能神经中合成的。
Bradykinin (10−8-10−5M) caused a concentration-dependent increase in cyclic GMP (cGMP) production in bovine tracheal smooth muscle in the absence of epithelium. The effect was calcium-dependent and was inhibited by pyrogallol (10 μM) and methylene blue (10 μM). The inhibition of pyrogallol was reversed by superoxide dismutase (100 Usnowml). Nitric oxide (NO) synthase inhibitors,NG-methyl-l-arginine (10–100 μM) andNG-nitro-l-arginine (10–100 μM) reduced cGMP accumulation induced by bradykinin in a concentration-dependent fashion, and the inhibition was reversed byl-arginine. Immunohistochemistry with a specific antibody against neuronal NO synthase from rat cerebellum showed positive staining localized in some nerve fibers. Bradykinin-induced cGMP accumulation appears to be related to the release of NO, part of which is probably synthesized in nonadrenergic noncholinergic nerve in bovine trachea.