Visualization of Proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection
Visualization of Proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection
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DOI：
10.1128/iai.70.1.389-394.2002
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发表时间：
2002-01-01
影响因子：
3.1
通讯作者：
Mobley, HLT
中科院分区：
文献类型：
--
作者：
Li, X;Zhao, H;Mobley, HLT
通过使用CBA小鼠导尿管尿路感染模型评估了尿路致病性奇异变形杆菌的尿素酶阴性突变株及其野生型亲本菌株的毒力。总体而言，插管小鼠比未插管小鼠更容易感染野生型奇异变形杆菌。在高接种量下，尿素酶阴性突变体成功地定殖于插管小鼠的膀胱，但不引起尿石症，并且其上升到肾脏的能力仍然严重减弱。使用共聚焦激光扫描显微镜和扫描电子显微镜，我们证明了在尿素酶诱导的结石基质中存在奇异变形杆菌。用茜素红S染色法检测奇异变形杆菌感染小鼠膀胱和肾组织中的钙沉积。
The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type P. mirabilis. At a high inoculum, the urease-negative mutant successfully colonized bladders of catheterized mice but did not cause urolithiasis and was still severely attenuated in its ability to ascend to kidneys. Using confocal laser scanning microscopy and scanning electron microscopy, we demonstrated the presence of P. mirabilis within the urease-induced stone matrix. Alizarin red S staining was used to detect calcium-containing deposits in bladder and kidney tissues of P. mirabilis-infected mice.