Inhibitory effect of skullcap (Scutellaria baicalensis) extract on ovalbumin permeation in vitro and in vivo
Inhibitory effect of skullcap (Scutellaria baicalensis) extract on ovalbumin permeation in vitro and in vivo
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DOI：
10.1016/j.foodchem.2013.01.042
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发表时间：
2013-09-15
期刊：
影响因子：
8.8
通讯作者：
Shon, Dong-Hwa
中科院分区：
文献类型：
--
作者：
Shin, Hee Soon;Bae, Min-Jung;Shon, Dong-Hwa
黄芩是一种广泛应用的膳食成分。本研究旨在揭示黄芩的新功能及其对肠上皮细胞过敏原渗透的作用机制。采用肠上皮Caco-2细胞单层，通过测定跨上皮电阻（TEER）和卵清蛋白（OVA）渗透量，评价黄芩对卵清蛋白（OVA）渗透的抑制作用。TEER增加和OVA流量下降，在剂量依赖性的方式，通过上调紧密连接相关的蛋白质与黄芩提取物浓度增加孵育的细胞。在体内研究中，来自口服摄入的蛋白的OVA的量在施用黄芩提取物时减少。首次发现黄芩提取物抑制卵清蛋白渗透的有效成分为黄芩素。这些发现表明，黄芩提取物可能通过抑制过敏原渗透在体外和体内减弱食物过敏反应。(C)2013爱思唯尔有限公司保留所有权利。
Scutellaria baicalensis Georgi (skullcap) has been widely used as a dietary ingredient. The purpose of this study was to reveal novel function of skullcap and its mechanism on allergen permeation in intestinal epithelial cells. Intestinal epithelial Caco-2 cell monolayers were used to evaluate the inhibitory effect of skullcap on ovalbumin (OVA) permeation by measuring transepithelial electrical resistance (TEER) and the quantity of permeated OVA. TEER increased and the OVA flux decreased in a dose-dependent manner through up-regulating tight junction-related proteins in cells incubated with increasing concentrations of skullcap extract. In the in vivo study, the amounts of OVA from orally ingested albumen reduced on administration of the skullcap extract. We also revealed for the first time that the active component of skullcap extract for inhibition of OVA permeation was baicalein. These findings demonstrated that skullcap extract might attenuate a food allergic response by inhibiting allergen permeation in vitro and in vivo. (C) 2013 Elsevier Ltd. All rights reserved.