Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
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DOI：
10.1016/j.jpba.2013.06.011
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发表时间：
2013-11-01
影响因子：
3.4
通讯作者：
Huang, Chenggang
中科院分区：
文献类型：
--
作者：
Ling, Yun;Li, Zhixiong;Huang, Chenggang
远志（Radix Polygalae，RP）为远志（Polygala tenuifolia Willd.）的干燥根，是一种众所周知的传统中药，对中枢神经系统具有镇静、抗精神病、改善认知、神经保护和抗炎治疗作用。建立了超高效液相色谱-电喷雾四极杆飞行时间串联质谱（UHPLC/ESI-Q-TOF-MS/MS）技术，用于大鼠口服远志后血浆和尿液中远志化学成分及其代谢产物的分离鉴定。样品在Agilent Zorbax Eclipse Plus-C18柱（100 mm x 2.1 mm，1.8 μ m）上分离，0.1%甲酸水溶液和乙腈作为移动的相，梯度条件下。总的来说，50个化合物，其特征在于从RP，其中9个是我们所知的第一次报告。在大鼠体内，血浆中检测到10种成分和2种代谢产物，尿液中检测到27种成分和7种代谢产物。这项工作的结果提高了我们对RP的化学成分及其代谢谱的理解。(C)2013爱思唯尔有限公司版权所有。
Radix Polygalae (RP), the dried root of Polygala tenuifolia Willd., is a well-known traditional Chinese medicine to mediate sedative, antipsychotic, cognitive improving, neuroprotective, and anti-inflammatory therapeutic effects on the central nervous system. In this work, ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) was established for the separation and characterization of the chemical constituents in Radix Polygalae and their metabolites in rat plasma and urine after oral administration. Samples were separated on an Agilent Zorbax Eclipse Plus-C18 column (100 mm x 2.1 mm, 1.8 mu m) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Overall, 50 compounds were characterized from the RP, 9 of which are to our knowledge reported for the first time. In vivo, 10 components and 2 metabolites were observed in rat plasma, and 27 components and 7 metabolites were detected in rat urine. The results from this work improve our understanding on the chemical constituents of RP and their metabolic profiling. (C) 2013 Elsevier B.V. All rights reserved.