Scopoletin Inhibits Rat Aldose Reductase Activity and Cataractogenesis in Galactose-Fed Rats
Scopoletin Inhibits Rat Aldose Reductase Activity and Cataractogenesis in Galactose-Fed Rats
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DOI：
10.1155/2013/787138
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Kim, Jin Sook
中科院分区：
文献类型：
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作者：
Kim, Junghyun;Kim, Chan-Sik;Kim, Jin Sook
白内障是人类失明的主要原因。醛糖还原酶（AR）是一种重要的限速酶，有助于糖尿病患者白内障的诱导。东莨菪素是巴山木兰花蕾中的主要活性成分，具有抑制AR活性的作用。为了评估东莨菪碱在体内减轻糖性白内障形成的能力，我们研究了其在饮食性半乳糖诱导的糖性白内障大鼠模型中的作用。半乳糖喂养的大鼠经口给予东莨菪碱（10或50 mg/kg体重），每天一次，持续2周。服用东茛菪亭可以延缓饮食半乳糖诱发的白内障的进展。东莨菪碱还可预防半乳糖诱导的透镜形态学变化，如透镜纤维肿胀和膜破裂。东莨菪碱对糖性白内障的保护作用是通过抑制AR活性和氧化应激介导的。这些结果表明，东莨菪碱是一种有效的治疗糖性白内障。
Cataracts are a major cause of human blindness. Aldose reductase (AR) is an important rate-limiting enzyme that contributes to cataract induction in diabetic patients. Scopoletin is the main bioactive constituent of flower buds from Magnolia fargesii and is known to inhibit AR activity. To assess scopoletin's ability to mitigate sugar cataract formation in vivo, we studied its effects in a rat model of dietary galactose-induced sugar cataracts. Galactose-fed rats were orally dosed with scopoletin (10 or 50 mg/kg body weight) once a day for 2 weeks. Administering scopoletin delayed the progression of the cataracts that were induced by dietary galactose. Scopoletin also prevented galactose-induced changes in lens morphology, such as lens fiber swelling and membrane rupture. Scopoletin's protective effect against sugar cataracts was mediated by inhibiting both AR activity and oxidative stress. These results suggest that scopoletin is a useful treatment for sugar cataracts.