Differential Expression of RDC1/CXCR7 in the Human Placenta
Differential Expression of RDC1/CXCR7 in the Human Placenta
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DOI：
10.1007/s10875-008-9258-4
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发表时间：
2009-05
影响因子：
9.1
通讯作者：
V. Tripathi;R. Verma;A. Dinda;N. Malhotra;J. Kaur;K. Luthra
中科院分区：
文献类型：
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作者：
V. Tripathi;R. Verma;A. Dinda;N. Malhotra;J. Kaur;K. Luthra
人类滋养层细胞和其他胎盘细胞表达的趋化因子受体对了解胎盘生长、发育的调控及其在母婴HIV传播中的作用具有重要意义。CXCR 7，现在是一种脱乙酰化的G蛋白偶联受体，最近已显示与配体ITAC和CXCL 12结合，已提出作为HIV-1、HIV-2和SIV株的共受体。CXCR 7在人胎盘组织中的差异表达尚未见报道。其中20个来自早期胎盘组织（8-10周龄）来自药物终止妊娠的胎盘和25个来自正常足月分娩的胎盘。PCR分析显示，与早期相比，足月人胎盘中CXCR 7的表达更高。这是进一步证实了real-timePCR.ConclusionOur研究揭示，为第一次，CXCR 7在早期（8-10周）和足月人胎盘的差异表达。CXCR 7在人类胎盘中的确切作用需要确定。据报告，艾滋病毒的垂直传播主要发生在妊娠末期。因此，我们在人类胎盘中发现CXCR 7表达增加，这保证了未来的研究，以评估其在HIV-1垂直传播中的作用。
IntroductionChemokine receptor expression by human trophoblast and other placental cells have important implications for understanding the regulation of placental growth, development, and their role in maternofetal HIV transmission. CXCR7, now a deorphanized G protein coupled receptor that has been recently shown to bind to the ligands ITAC and CXCL12 has been proposed to act as a co-receptor for HIV-1, HIV-2, and SIV strains. The differential expression of CXCR7 in the human placenta is not yet reported.MethodsThe expression of CXCR7 was studied in 45 different human placental tissues, of which 20 were from early placental tissues (8–10 week old) obtained from medically terminated pregnancies and 25 were placenta from normal term deliveries.ResultsImmunohistochemistry and RT-PCR analysis revealed a greater expression of CXCR7 in term human placenta as compared to the early stage. This was further confirmed by real-time PCR.ConclusionOur study reveals, for the first time, the differential expression of CXCR7 in early (8–10 weeks) and term human placenta. The precise role of CXCR7 in the human placenta needs to be determined. HIV vertical transmission is reported to occur mainly during the end stages of pregnancy. Our finding of increased CXCR7 expression in the term human placenta therefore warrants future studies to assess its role in the vertical transmission of HIV-1.