Herb pair of Ephedrae Herba-Armeniacae Semen Amarum alleviates airway injury in asthmatic rats
Herb pair of Ephedrae Herba-Armeniacae Semen Amarum alleviates airway injury in asthmatic rats
复制标题
麻黄-杏仁对缓解哮喘大鼠气道损伤
DOI：
10.1016/j.jep.2020.113745
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发表时间：
2021-01-04
影响因子：
5.4
通讯作者：
Li, Wen-hong
中科院分区：
文献类型：
--
作者：
Ma, Jia-xin;Xiao, Xiong;Li, Wen-hong
民族药理学相关性：麻黄（Ephedra sinica Stapf.）和苦杏仁（Armeniacum Semen Amarum，阿萨，Prunus armeniaca L.）变种ansu Maxim.）本研究的目的：探讨EH与阿萨配伍（EAC）减轻哮喘大鼠气道损伤的最佳比例及其可能的机制。方法：大鼠在致敏前1 h用氯化乙酰胆碱和二磷酸组胺的混合物致敏，地塞米松或生理盐水7天。结果：透射电镜观察：EAC（C、E组）对哮喘大鼠气道上皮细胞超微结构的修复作用最显著，而对哮喘大鼠气道上皮细胞凋亡的影响最显著。TUNEL：地塞米松和EAC（B、C、E、F组）抑制哮喘大鼠气道上皮细胞凋亡（P < 0.05）。原位杂交：EAC（E组）抑制EGFR和Bcl-2 mRNA的过表达免疫印迹法：EAC A、B、C、E、F组气道上皮EGFR和Bcl-2蛋白表达均明显低于对照组结论：EAC能抑制气道上皮结构的异常改变和细胞凋亡，从而减轻气道损伤。本研究中，EH与阿萨减轻哮喘大鼠气道上皮损伤的最佳组合为E组（EH：阿萨= 8：4.5）。
Ethnopharmacological relevance: Ephedrae Herba (EH, Ephedra sinica Stapf.) and Armeniacae Semen Amarum (ASA, Prunus armeniaca L. var. ansu Maxim.) have been used to treat asthma, cold, fever, and cough in China for thousands of years.Aim of the study: In this study, we aimed to investigate the optimal ratio of EH and ASA compatibility (EAC) to reduce airway injury in asthmatic rats and its possible mechanism.Methods: Rats were sensitized with a mixture of acetylcholine chloride and histamine bisphosphate 1 h before sensitization by intragastric administration of EAC or dexamethasone or saline for 7 days. Subsequently, the ultrastructure of rat airway epithelial tissue changes, apoptosis of the airway epithelial cells, and the expression of mRNA and protein of EGRF and Bcl-2 were detected.Results: Transmission electron microscope: EAC (groups C and E) had the most prominent effect on repairing airway epithelial cells' ultrastructural changes in asthmatic rats. TUNEL: dexamethasone and EAC (groups B, C, E and F) inhibited the apoptosis of airway epithelial cells in asthmatic rats (P < 0.05). In situ hybridization: EAC (group E) inhibited the overexpression of EGFR and Bcl-2 mRNA (P < 0.05).Western Blotting: EAC (groups A, B, C, E and F) inhibited the upregulation of airway epithelial EGFR and Bcl-2 protein expression (P < 0.01).Conclusions: Our findings indicate that EAC can inhibit abnormal changes in airway epithelial structure and apoptosis of airway epithelial cells, thereby alleviating airway injury. In this study, the best combination of EH and ASA to alleviate airway epithelial injury in asthmatic rats was group E (EH: ASA = 8: 4.5).