Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes.
Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes.
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DOI：
10.1186/s12864-017-3751-1
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发表时间：
2017-05-11
期刊：
影响因子：
4.4
通讯作者：
Wintermantel WM
中科院分区：
文献类型：
--
作者：
Kaur N;Chen W;Zheng Y;Hasegawa DK;Ling KS;Fei Z;Wintermantel WM
粉虱威胁着世界各地的农作物生产，是多食性的，并传播数百种植物病毒。由于以感染了半持久性传播病毒的植物为食，粉虱的基因表达如何改变，目前还知之甚少。番茄褪绿病病毒是由烟粉虱传播的一种半持续型病毒，侵染包括番茄在内的多种全球重要的农业和观赏作物。为了确定烟粉虱在取食感染CriniVirus(ToCV)的番茄植株后全球基因调控的变化，利用RNA-Seq技术对感染和未感染的番茄在24、48和72小时获取访问期后的烟粉虱种群进行了比较转录分析。取食侵染ToCV番茄的烟粉虱和取食未感染番茄的烟粉虱在3个取食时间段的基因表达差异显著：取食24 h时为447个上调基因和542个下调基因，48 h时为4个上调基因和7个下调基因，72 h时为50个上调基因和160个下调基因。分析表明，与代谢途径、信号转导、转运和分解代谢、受体、葡萄糖转运蛋白、α-葡萄糖苷酶和尿酸途径相关的基因表达存在差异，同时还发现了大量差异表达的新的孤儿基因。结果首次证明了烟粉虱对感染了半持久性传播病毒的寄主植物的特异性和时间调节的反应，并促进了对促进病毒传播的烟粉虱媒介-病毒相互作用的理解。烟粉虱传播半持久性病毒被认为需要病毒及其媒介之间的特定相互作用，使病毒颗粒与烟粉虱口器内的因子结合。这些结果有助于更广泛地了解烟粉虱传播CriniVirus的潜在机制，有助于识别烟粉虱中影响病毒相互作用或传播的基因或基因，从而促进基于遗传学的新型控制方法的开发。本文的在线版本(doi：10.1186/s12864-0173751-1)包含补充材料，授权用户可以使用。
Whiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato. To determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission. Whitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses. The online version of this article (doi:10.1186/s12864-017-3751-1) contains supplementary material, which is available to authorized users.