Tandem cytochrome P450 monooxygenase genes and splice variants in the white rot fungus Phanerochaete chrysosporium:: cloning, sequence analysis, and regulation of differential expression
Tandem cytochrome P450 monooxygenase genes and splice variants in the white rot fungus Phanerochaete chrysosporium:: cloning, sequence analysis, and regulation of differential expression
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DOI：
10.1016/s1087-1845(02)00508-x
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发表时间：
2003-02-01
影响因子：
3
通讯作者：
Mishra, PK
中科院分区：
文献类型：
--
作者：
Yadav, JS;Soellner, MB;Mishra, PK
Phanerochaete chrysosporium, the model white rot fungus, uses P450 monooxygenation for oxidation of xenobiotic chemicals. To characterize its P450 systems, we have isolated two tandemly linked full-length P450 monooxygenase genes, pc-1 (2510 bp) and pc-2(2503 bp). cDNA sequence and splice site analysis showed multiple small putative introns (35-82 bp), 17 in pc-1 and 16 in pc-2. The deduced P450 proteins PC-I (531 aa, MW 60,070) and PC-2 (538 aa, MW 61,765) showed 55%, aa identity with each other and low relatedness (25-36%) with P450 gene families CYP52, CYP86, CYP4, and CYP3 decreasing in that order. Alternate splicing was identified for pc-1 transcription. Real-time quantitative RT-PCR analysis on 4-day cultures showed an elevated level of expression (similar to8-fold) of pc-1 in nutrient-limited (ligninolytic) as compared to nutrient-rich (non-ligninolytic) conditions. Interestingly, the pc-1 gene is expressible in both primary and secondary phases of growth with peak expression occurring with secondary metabolism. (C) 2002 Elsevier Science (USA). All rights reserved.
