Deep evolutionary origin of limb and fin regeneration
Deep evolutionary origin of limb and fin regeneration
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DOI：
10.1073/pnas.1900475116
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发表时间：
2019-07-23
影响因子：
11.1
通讯作者：
Schneider, Igor
中科院分区：
文献类型：
--
作者：
Darnet, Sylvain;Dragalzew, Aline C.;Schneider, Igor
蝾螈和肺鱼是唯一能够再生成对附肢的肉鳍动物（叶鳍脊椎动物），无论截肢水平如何。在辐鳍鱼类中，只有在多鳍类鱼类中才发现鳍内骨骼截肢后的再生。这种能力是否在肉蟹猴和放线菌中独立进化，或者有一个共同的起源仍然未知。在这里，我们结合联合收割机鳍再生测定和比较RNA测序（RNA-seq）分析的Polypterus和蝾螈芽基提供支持的共同起源的成对附肢再生硬骨鱼（骨脊椎动物）。我们发现，除了poly-pterids，再生后鳍内骨骼截肢发生在现存的代表2其他nonteleost放线菌：美国白鲟（Chondrostei）和斑点雀鳝（Holostei）。此外，我们评估了再生的硬骨鱼4种，并表明，与蓝色的古鱼（Anabantidae），3种能够再生鳍内骨骼截肢后：白色罪犯和奥斯卡（慈鲷科），和金鱼（鲤科）。我们的比较RNA-seq分析的再生芽的蝾螈和Polypterus揭示了共同的遗传途径和表达谱的激活，与附件再生的共享遗传程序一致。来自早期Polypterus blastema的RNA-seq数据与来自蝾螈肢芽和肢再生阶段的单细胞RNA-seq数据的比较表明，Polypterus和蝾螈共享再生特异性遗传程序。总的来说，我们的研究结果支持一个深层次的进化起源的成对附肢再生硬骨鱼类，并提供了一个进化框架的研究附肢再生的遗传基础。
Salamanders and lungfishes are the only sarcopterygians (lobe-finned vertebrates) capable of paired appendage regeneration, regardless of the amputation level. Among actinopterygians (ray-finned fishes), regeneration after amputation at the fin endoskeleton has only been demonstrated in polypterid fishes (Cladistia). Whether this ability evolved independently in sarcopterygians and actinopterygians or has a common origin remains unknown. Here we combine fin regeneration assays and comparative RNA-sequencing (RNA-seq) analysis of Polypterus and axolotl blastemas to provide support for a common origin of paired appendage regeneration in Osteichthyes (bony vertebrates). We show that, in addition to poly-pterids, regeneration after fin endoskeleton amputation occurs in extant representatives of 2 other nonteleost actinopterygians: the American paddlefish (Chondrostei) and the spotted gar (Holostei). Furthermore, we assessed regeneration in 4 teleost species and show that, with the exception of the blue gourami (Anabantidae), 3 species were capable of regenerating fins after endoskeleton amputation: the white convict and the oscar (Cichlidae), and the gold-fish (Cyprinidae). Our comparative RNA-seq analysis of regenerating blastemas of axolotl and Polypterus reveals the activation of common genetic pathways and expression profiles, consistent with a shared genetic program of appendage regeneration. Comparison of RNA-seq data from early Polypterus blastema to single-cell RNA-seq data from axolotl limb bud and limb regeneration stages shows that Polypterus and axolotl share a regeneration-specific genetic program. Collectively, our findings support a deep evolutionary origin of paired appendage regeneration in Osteichthyes and provide an evolutionary framework for studies on the genetic basis of appendage regeneration.