Three-dimensional structure determination of gold nanotriangles in solution using X-ray free-electron laser single-particle analysis
Three-dimensional structure determination of gold nanotriangles in solution using X-ray free-electron laser single-particle analysis
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DOI：
10.1364/optica.457352
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发表时间：
2022-07-20
期刊：
影响因子：
10.4
通讯作者：
Tama, Florence
中科院分区：
文献类型：
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作者：
Nakano, Miki;Miyashita, Osamu;Tama, Florence
x射线自由电子激光器（XFELs）已经允许在接近生理条件下对纳米级样品进行成像。为了实现三维（3D）纳米结构重建，需要解决许多挑战，例如数据采集的样品传输和噪声衍射图样的数据处理。在这里，我们展示了使用微液体外壳阵列（mlea）样品支架在SPring-8埃紧凑自由电子激光器上测量的XFEL衍射数据对金纳米粒子的三维结构重建。mlea可以在溶液中进行测量，但是，它们会引起大量的背景噪声。因此，我们进行了一系列的数据分析，以确定适合三维重建的衍射模式，以及用于估计背景噪声的非命中模式。数据的背景减法显著提高了恢复结构的质量，使用傅里叶壳相关估计分辨率为5 nm。我们的论文揭示了使用mlea结合开发的数据分析协议进行XFEL成像的显着潜力。(C) 2022光学出版集团
X-ray free-electron lasers (XFELs) have allowed the imaging of nanoscale samples in near-physiological conditions. To achieve three-dimensional (3D) nanostructural reconstruction, many challenges need to be addressed, such as sample delivery for data collection and data processing of noisy diffraction patterns. Here, we provided a demonstration of the 3D structure reconstruction of a gold nanoparticle from XFEL diffraction data measured at the SPring-8 Angstrom Compact Free-Electron Laser using microliquid enclosure arrays (MLEAs) sample holders. MLEAs enable in-solution measurements, however, they induce a significant amount of background noise. Thus, we performed a series of data analyses to identify the diffraction patterns suitable for 3D reconstruction as well as nonhit patterns to estimate the background noise. The background subtraction from the data significantly improved the quality of the restored structure, with the resolution estimated to be 5 nm using Fourier shell correlation. Our paper has revealed the notable potential of XFEL imaging using MLEAs in combination with the developed data-analysis protocol. (C) 2022 Optica Publishing Group