Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures
Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures
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DOI：
10.1016/s0142-9612(00)00350-1
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发表时间：
2001-05-01
期刊：
影响因子：
14
通讯作者：
Bellamkonda, RV
中科院分区：
文献类型：
--
作者：
Balgude, AP;Yu, X;Bellamkonda, RV
优化神经突起延伸支架的力学性能是神经组织工程的关键。琼脂糖水凝胶可用于体外刺激和维持初级感觉神经节的三维神经突延伸。本研究旨在探讨背根神经节（DRG）神经突起延伸与琼脂糖凝胶力学性质之间的结构-功能关系。产生一系列不同浓度的琼脂糖凝胶，并测量E9 DRG神经突延伸的相应速率。在0.75-2.00%（wt/vol）凝胶浓度范围内，神经突延伸率与琼脂糖凝胶的机械刚度呈负相关。此外，我们假设一个物理模型，预测在琼脂糖凝胶中的神经突延伸率，如果凝胶刚度是一个已知的参数。这个模型是基于Heidemann和Buxbaum的神经突延伸模型。这些结果，如果扩展到其他形态和化学特征的支架，将有助于显着的三维支架的神经组织工程的设计标准。(C)2001爱思唯尔科技有限公司版权所有。
The optimization of scaffold mechanical properties for neurite extension is critical for neural tissue engineering. Agarose hydrogels can be used to stimulate and maintain three-dimensional neurite extension from primary sensory ganglia in vitro. The present study explores the structure-function relationship between dorsal root ganglion (DRG) neurite extension and agarose gel mechanical properties. A range of agarose gels of differing concentrations were generated and the corresponding rate of E9 DRG neurite extension was measured. Rate of neurite extension was inversely correlated to the mechanical stiffness of agarose gels in the range of 0.75-2.00% (wt/vol) gel concentrations. In addition, we postulate a physical model that predicts the rate of neurite extension in agarose gels, if gel stiffness is a known parameter. This model is based on Heidemann and Buxbaum's model of neurite extension. These results, if extended to scaffolds of other morphological and chemical features, would contribute significantly to the design criteria of three-dimensional scaffolds for neural tissue engineering. (C) 2001 Elsevier Science Ltd. All rights reserved.