Tissue-engineering of vascular grafts containing endothelium and smooth-muscle using triple-coaxial cell printing
Tissue-engineering of vascular grafts containing endothelium and smooth-muscle using triple-coaxial cell printing
复制标题
利用三轴向细胞打印技术，对含有内皮细胞和平滑肌的血管移植物进行组织工程改造
DOI：
10.1063/1.5099306
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发表时间：
2019-12-01
影响因子：
15
通讯作者：
Cho, Dong-woo
中科院分区：
文献类型：
--
作者：
Gao, Ge;Kim, Hyeok;Cho, Dong-woo
组织工程学已经成为一种有希望的方法，可以用于治疗心血管疾病的可行的小直径血管移植物。构建这类血管的一个挑战是对内皮细胞和平滑肌细胞进行适当的定位，以及促进它们的细胞功能以产生功能组织。到目前为止，构建具有内皮组织和肌肉组织的小直径血管替代物对于移植物获得抗血栓功能和足够的强度以避免血栓形成和承受血压是必不可少的，但尚未被证明。在这项研究中，我们设计了包含功能内皮层和肌细胞层的小直径血管移植物，这已在活体大鼠模型中得到证实。我们构建的血管移植物使用血管组织衍生的细胞外基质生物墨水和水库辅助的三同轴细胞打印技术。在一项概念验证研究中，将早熟的血管作为大鼠腹主动脉的移植物植入三周，在研究结束时，所有植入物都显示出良好的通畅性、完整的内皮、重塑的平滑肌以及与宿主组织的整合。这些结果表明，我们的组织工程化仿生血管的方法为构建耐用的小直径血管移植物提供了一条有希望的途径，这些移植物可能会用于未来的心血管疾病治疗。由AIP出版公司授权出版。
Tissue engineering has emerged as a promising approach to viable small-diameter vascular grafts that may be used to treat cardiovascular diseases. One challenge in constructing such blood vessels is proper localization of endothelial cells and smooth muscle cells, as well as promotion of their cellular functions to generate functional tissues. Thus far, construction of small-diameter vascular substitutes with both endothelial and muscular tissues, which is essential for the grafts to acquire antithrombosis function and sufficient strength to avoid thrombus formation as well as to withstand blood pressure, has not yet been demonstrated. In this study, we engineer small-diameter blood vessel grafts containing both functional endothelial and muscular cell layers, which has been demonstrated in vivo in a living rat model. Our construction of the blood vessel grafts uses vascular-tissue-derived extracellular matrix bioinks and a reservoir-assisted triple-coaxial cell printing technique. The prematured vessel was implanted for three weeks as a graft of rat abdominal aorta in a proof-of-concept study where all implants showed great patency, intact endothelium, remodeled smooth muscle, and integration with host tissues at the end of the study. These outcomes suggest that our approach to tissue-engineered biomimetic blood vessels provides a promising route for the construction of durable small-diameter vascular grafts that may be used in future treatments of cardiovascular diseases. Published under license by AIP Publishing.