New exoelectrogen Citrobacter sp SX-1 isolated from a microbial fuel cell
New exoelectrogen Citrobacter sp SX-1 isolated from a microbial fuel cell
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DOI：
10.1111/j.1365-2672.2011.05129.x
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发表时间：
2011-11-01
影响因子：
4
通讯作者：
Liu, H.
中科院分区：
文献类型：
--
作者：
Xu, S.;Liu, H.
目的：从微生物燃料电池（MFC）中分离、鉴定和表征一株新的产电细菌。方法和结果：在厌氧条件下，以柠檬酸铁为电子受体，采用常规平板法从无介体MFC中分离到一株产电细菌SX-1。系统发育分析表明，该菌株与柠檬酸杆菌属（Citrobacter）的成员有亲缘关系，其中与柠檬酸杆菌属（Citrobacter sp. sdy-48）的亲缘关系最近。菌株SX-1在MFC中以柠檬酸盐、乙酸盐、葡萄糖、蔗糖、甘油和乳糖为电解质产生电流，最高电流密度为205 mA m（-2）。循环伏安分析表明，膜相关蛋白在促进电子从细菌向电极传递中可能起着重要作用。结论：本研究首次证明柠檬酸杆菌属细菌可以将电子传递给胞外电子受体。柠檬酸杆菌SX-1菌株能够在MFC中从广泛的底物产生电。研究的意义和影响：这一发现增加了已知的发电外生产电菌的多样性，并提供了一个新的菌株来探索细胞外电子从细菌到电极的转移机制。SX-1对基质的广泛利用增加了MFC在可再生能源发电和废物处理方面的应用潜力。
Aims: Isolation, identification and characterization of a new exoelectrogenic bacterium from a microbial fuel cell (MFC).Methods and Results: Exoelectrogenic bacterial strain SX-1 was isolated from a mediator-less MFC by conventional plating techniques with ferric citrate as electron acceptor under anaerobic condition. Phylogenetic analysis of the 16S rDNA sequence revealed that it was related to the members of Citrobacter genus with Citrobacter sp. sdy-48 being the most closely related species. The bacterial strain SX-1 produced electricity from citrate, acetate, glucose, sucrose, glycerol and lactose in MFCs with the highest current density of 205 mA m(-2) generated from citrate. Cyclic voltammetry analysis indicated that membrane-associated proteins may play an important role in facilitating the electrons transferring from bacteria to electrode.Conclusions: This is the first study that demonstrates that Citrobacter species can transfer electrons to extracellular electron acceptors. Citrobacter strain SX-1 is capable of generating electricity from a wide range of substrates in MFCs.Significance and Impact of the Study: This finding increases the known diversity of power generating exoelectrogens and provided a new strain to explore the mechanisms of extracellular electron transfer from bacteria to electrode. The wide range of substrate utilization by SX-1 increases the application potential of MFCs in renewable energy generation and waste treatment.