EXTENDED CHROMATIN FIBRES: CRYSTALLINITY, MOLECULAR ORDER AND REACTIVITY TO CONCANAVALIN‐A
EXTENDED CHROMATIN FIBRES: CRYSTALLINITY, MOLECULAR ORDER AND REACTIVITY TO CONCANAVALIN‐A
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延长的染色质纤维：结晶度、分子顺序和刀豆蛋白-A 的反应性
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
C. Vidal
中科院分区：
文献类型：
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作者：
C. Vidal
本研究的目的是测试延长染色质纤维（ECF）、微球和超微球的可重复性形成，并通过这些结构的光学各向异性检测分子秩序和结晶度。鸡红细胞涂片和小鼠肝细胞印迹分别用1% Triton - X100溶液中2.0-3.0 m NaCl溶液垂直处理，然后用0.025%甲苯胺蓝在pH4下染色。甲苯胺蓝与DNA结合后的双折射检测和双折射异常色散（ADB）的颜色显示，在ECF形成后，脱氧和浓缩染色质中的DNA分子秩序和结晶度得以保留。Con‐A与糖蛋白甘露糖/葡萄糖残基的结合试验在细胞核内、ECF内、珠粒和超珠粒中得到证实。ECF的形成不规律。块状细胞未显示出ECF，尽管在细胞核内观察到染色质的流动性。电镜观察表明，经0.77 m NaCl处理后，ECF始终从核中向外扩散，在块状核中纤维聚集而不是扩散。
The present study was undertaken to test the reproducible formation of the extended chromatin fibres (ECF), beads and superbeads, and detect molecular order and crystallinity by optical anisotropies in those structures. Chicken erythrocyte smears and mouse liver cell imprints were treated with 2.0–3.0 m NaCl solution in 1% Triton‐X100 vertically prior to staining with 0.025% toluidine blue at pH4. Detection of birefringence and colours of abnormal dispersion of birefringence (ADB) following toluidine blue binding to DNA revealed that the DNA molecular order and crystallinity in decondensed and condensed chromatin are preserved after ECF formation. The tests for Con‐A binding to mannose/glucose residues of glycoproteins was confirmed within nuclei, and in the ECF, beads and superbeads. ECF formation was not regular. Clumped cells did not show ECF, although chromatin mobility was observed within the nuclei. Electron microscopy demonstrated that after treatment of nuclei with 0.77 m NaCl ECF always spread from the nuclei, in clumped nuclei the fibres aggregated instead of spreading.
影响因子：
56.9
作者：
Perkins, TT;Smith, DE;Chu, S
通讯作者：
Chu, S