Toward a global analysis of the human pituitary proteome by multiple gel-based technology.
Toward a global analysis of the human pituitary proteome by multiple gel-based technology.
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DOI：
10.1021/ac050354e
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发表时间：
2005-08
影响因子：
7.4
通讯作者：
Yingxin Zhao;F. Giorgianni;D. Desiderio;Bing Fang;S. Beranova‐Giorgianni
中科院分区：
文献类型：
--
作者：
Yingxin Zhao;F. Giorgianni;D. Desiderio;Bing Fang;S. Beranova‐Giorgianni
We describe a comprehensive method for the analysis of complex proteomes, multiple gel-based technology (MGT), which combines in-gel IEF-LC-MS/MS and SDS-PAGE-LC-MS/MS strategies. MGT was applied to the analysis of the proteome of human pituitary tissue. A total of 1449 proteins were uniquely identified. To our knowledge, this is the most comprehensive proteomic analysis of human pituitary tissue to date. Categorization of the identified proteins revealed that MGT provides an excellent and largely unbiased access to proteins with diverse characteristics, including low-abundance proteins, membrane proteins, and proteins with extremes in pI and MW.
