Exclusive Liquid Repellency: An Open Multi-Liquid-Phase Technology for Rare Cell Culture and Single-Cell Processing
Exclusive Liquid Repellency: An Open Multi-Liquid-Phase Technology for Rare Cell Culture and Single-Cell Processing
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DOI：
10.1021/acsami.8b03627
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发表时间：
2018-05-23
影响因子：
9.5
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
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作者：
Li, Chao;Yu, Jiaquan;Beebe, David J.
多液相系统（例如，油背景中的水滴）的高驱液性概念已应用于生物医学研究领域，以实现单液相系统所不具备的固有优势。这些优点包括最大限度地减少分析物损失，易于操作单细胞样品，消除生物污垢，以及易于加载和提取样品。在本文中，我们提出了预测固液液体系润湿性的广义设计规则（特别是区分纯液体驱避和有限液体驱避），以扩展纯液体驱避的应用。然后，我们将ELR应用于细胞生物学中具有开放微流控设计的两个模型系统：(1)哺乳动物细胞的原位底油培养和组合共培养，以证明定向单细胞多封装，与随机细胞播种相比，样品浪费最少；(2)分离纯循环肿瘤细胞群，这是某些下游分析所需要的，包括测序和基因表达谱。
The concept of high liquid repellency in multi-liquid-phase systems (e.g., aqueous droplets in an oil background) has been applied to areas of biomedical research to realize intrinsic advantages not available in single-liquid-phase systems. Such advantages have included minimizing analyte loss, facile manipulation of single-cell samples, elimination of biofouling, and ease of use regarding loading and retrieving of the sample. In this paper, we present generalized design rules for predicting the wettability of solid liquid liquid systems (especially for discrimination between exclusive liquid repellency (ELR) and finite liquid repellency) to extend the applications of ELR. We then apply ELR to two model systems with open microfluidic design in cell biology: (1) in situ underoil culture and combinatorial coculture of mammalian cells in order to demonstrate directed single-cell multiencapsulation with minimal waste of samples as compared to stochastic cell seeding and (2) isolation of a pure population of circulating tumor cells, which is required for certain downstream analyses including sequencing and gene expression profiling.