Investigating the Effects of Stove Emissions on Ocular and Cancer Cells
Investigating the Effects of Stove Emissions on Ocular and Cancer Cells
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DOI：
10.1038/s41598-019-38803-4
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发表时间：
2019-02-12
影响因子：
4.6
通讯作者：
Biswas, Pratim
中科院分区：
文献类型：
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作者：
Karakocak, Bedia Begum;Patel, Sameer;Biswas, Pratim
世界上超过三分之一的人口依靠固体燃料做饭和取暖，这对健康造成了重大影响。虽然已知固体燃料燃烧排放物会增加慢性阻塞性肺病和肺癌等疾病的患病率，但其对眼睛的影响尚未得到充分研究。本研究评估了固体燃料燃烧排放物对健康眼细胞和癌细胞系的急性毒性。三个健康的眼细胞系（角膜，透镜，和视网膜上皮细胞）和癌细胞系（中国仓鼠卵巢细胞）暴露于液相和气相排放的苹果木和煤炭燃烧。暴露后，用电池阻抗谱法监测实时细胞附着行为至少120小时。分别用MTT、ApoTox-Glo和ROS-Glo H2 O2测定法定量细胞的活力、凋亡细胞的量和活性氧（ROS）的产生。结果表明，煤炭排放损害的生存能力的眼细胞比苹果木排放。有趣的是，尽管癌细胞的活力没有受到影响，但它们产生的ROS是健康细胞的1.7至2.7倍。这项急性暴露研究提供了令人信服的证据，证明生物质燃烧排放损害眼细胞的活力，并增加ROS的产生。增加的ROS产生对眼细胞是致命的，但它促进了癌细胞的生长。
More than a third of the world's population relies on solid fuels for cooking and heating, with major health consequences. Although solid fuel combustion emissions are known to increase the prevalence of illnesses such as chronic obstructive pulmonary disease and lung cancer, however, their effect on the eyes is underexplored. This study assesses the acute toxicity of solid fuel combustion emissions on healthy ocular cells and a cancer cell line. Three healthy ocular cell lines (corneal, lens, and retinal epithelial cells) and a cancer cell line (Chinese hamster ovary cells) were exposed to liquid and gas phase emissions from applewood and coal combustion. Following the exposure, real-time cell attachment behavior was monitored for at least 120 hours with electrical cell impedance spectroscopy. The viability of the cells, amount of apoptotic cells, and generation of reactive oxygen species (ROS) were quantified with MTT, ApoTox-Glo, and ROS-Glo H2O2 assays, respectively. The results showed that coal emissions compromised the viability of ocular cells more than applewood emissions. Interestingly, the cancer cells, although their viability was not compromised, generated 1.7 to 2.7 times more ROS than healthy cells. This acute exposure study provides compelling proof that biomass combustion emissions compromise the viability of ocular cells and increase ROS generation. The increased ROS generation was fatal for ocular cells, but it promoted the growth of cancer cells.