A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus
A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona Virus
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DOI：
10.1002/adhm.201600334
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发表时间：
2016-09-07
影响因子：
10
通讯作者：
Lee, Hyukjin
中科院分区：
文献类型：
--
作者：
Jung, Il Young;You, Jae Bem;Lee, Hyukjin
Trail聚合可以显著增强DhITACT系统。DhITACT-Trail（互补靶迹聚合等温扩增形成的DNA水凝胶）提供了假血清标本中靶RNA链的可靠诊断。与传统分析相比，该系统需要最少的液体处理。此外，通过光学检测可在30分钟内获得明确的诊断结果。
Trail polymerization enables a significant enhancement of the DhITACT system. DhITACT-Trail (DNA hydrogel formation by isothermal amplification of complementary targets trail polymerization) offers a robust diagnosis of target RNA strands in pseudo-serum specimen. This system requires minimum liquid handling as compared to conventional analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.