INDUCTION OF ANTHOCYANIN ACCUMULATION BY CYTOKININS IN ARABIDOPSIS-THALIANA
INDUCTION OF ANTHOCYANIN ACCUMULATION BY CYTOKININS IN ARABIDOPSIS-THALIANA
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DOI：
10.1104/pp.108.1.47
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发表时间：
1995-05-01
期刊：
影响因子：
7.4
通讯作者：
HAMMER, PE
中科院分区：
文献类型：
--
作者：
DEIKMAN, J;HAMMER, PE
用外源细胞分裂素处理的拟南芥植株积累花青素。我们的特点是这种反应，因为它是潜在的有用的细胞分裂素反应的遗传标记。花色素苷生物合成途径的四个基因，苯丙氨酸解氨酶1（PAL 1），查尔酮合酶（CHS），查尔酮异构酶（CHI），和二氢黄酮醇还原酶（DFR）的mRNA水平被证明协同增加响应苄基腺嘌呤（BA）。然而，核连续转录实验表明，虽然CHS和DFR在转录水平上由BA控制，PAL 1和CHI在转录后由BA控制。CHS mRNA水平在喷施BA后2h内升高，3h达到高峰。PAL 1 mRNA水平在6 h内没有增加。我们还发现，PAL 1，CHS，CHI和DFR mRNA水平在24小时内波动，似乎是由生物钟控制。讨论了细胞分裂素调控与光调控CHS基因转录的关系。
Arabidopsis thaliana plants treated with exogenous cytokinins accumulate anthocyanin pigments. We have characterized this response because it is potentially useful as a genetic marker for cytokinin responsiveness. Levels of mRNAs for four genes of the anthocyanin biosynthesis pathway, phenylalanine ammonia lyase 1 (PAL1), chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR) were shown to increase coordinately in response to benzyladenine (BA). However, nuclear run-on transcription experiments suggested that although CHS and DFR are controlled by BA at the transcriptional level, PAL1 and CHI are controlled by BA posttranscriptionally. CHS mRNA levels increased within 2 h of BA spray application, and peaked by 3 h. Levels of PAL1 mRNA did not increase within 6 h of BA spray. We also showed that PAL1, CHS, CHI, and DFR mRNA levels fluctuate during a 24-h period and appear to be controlled by a circadian clock. The relation between cytokinin regulation and light regulation of CHS gene transcription is discussed.