Dietary protein restriction decreases oxidative protein damage, peroxidizability index, and mitochondrial complex I content in rat liver
Dietary protein restriction decreases oxidative protein damage, peroxidizability index, and mitochondrial complex I content in rat liver
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DOI：
10.1093/gerona/62.4.352
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发表时间：
2007-04-01
影响因子：
5.1
通讯作者：
Pamplona, Reinald
中科院分区：
文献类型：
--
作者：
Ayala, Victoria;Naudi, Alba;Pamplona, Reinald
热量限制（CR）减少氧化损伤，这有助于减缓衰老速度。目前尚不清楚这种减少是由于卡路里本身还是特定的饮食成分。在这项工作中，Wistar大鼠的蛋白质摄入量比对照组减少了40%。7周后，蛋白质限制（PR）动物的肝脏显示氧化蛋白损伤，膜不饱和度和线粒体复合物I含量降低。结果和先前的信息表明，PR诱导的衰老速率的降低可能部分是由于线粒体活性氧产生和DNA和蛋白质氧化修饰的减少，脂肪酸组分的增加更能抵抗氧化损伤，以及复合物I的表达减少，类似于CR期间发生的情况。最近的研究表明，PR的这些好处可能是由饮食控制降低蛋氨酸摄入量引起的。
Caloric restriction (CR) decreases oxidative damage, which contributes to the slowing of aging rate. It is not known if such decreases are due to calories themselves or specific dietary components. In this work, the ingestion of proteins of Wistar rats was decreased by 40% below that of controls. After 7 weeks, the liver of the protein-restricted (PR) animals showed decreases in oxidative protein damage, degree of membrane unsaturation, and mitochondrial complex I content. The results and previous information suggest that the decrease in the rate of aging induced by PR can be due in part to decreases in mitochondrial reactive oxygen species production and DNA and protein oxidative modification, increases in fatty acid components more resistant to oxidative damage, and decreased expression of complex I, analogously to what occurs during CR. Recent studies suggest that those benefits of PR could be caused, in turn, by the lowered methionine intake of that dietary manipulation.