Induction of apoptosis by eicosapentaenoic acid in esophageal squamous cell carcinoma.
Induction of apoptosis by eicosapentaenoic acid in esophageal squamous cell carcinoma.
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发表时间：
2014-12
影响因子：
2
通讯作者：
K. Mizoguchi;H. Ishiguro;M. Kimura;Hiroki Takahashi;N. Sakamoto;Tatsuya Tanaka;H. Takeyama
中科院分区：
文献类型：
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作者：
K. Mizoguchi;H. Ishiguro;M. Kimura;Hiroki Takahashi;N. Sakamoto;Tatsuya Tanaka;H. Takeyama
背景技术二十碳五烯酸(EPA)抑制来自结肠癌、胰腺癌、乳腺癌和其他癌症的细胞系的增殖。很少有报道描述 EPA 对食道癌细胞系的影响。材料和方法我们通过 WST-1 测定研究了 EPA 对食管鳞状细胞癌细胞系 TE11 和 KYSE180 增殖的影响。通过 DNA 片段化分析评估细胞凋亡。通过蛋白质印迹分析评估凋亡相关蛋白（caspase-3、-7、-9 和聚（ADP-核糖）聚合酶（PARP））和裂解的 caspase-3、-7、-9 和 PARP 的水平。结果暴露于EPA 24小时后，KYSE180和TE11细胞增殖以剂量依赖性方式受到抑制（p<0.05）。此外，半胱天冬酶-3、-7、-9和PARP被激活。通过 DNA 碎片分析检测，EPA（0.1 μM、1 μM、10 μM）以剂量依赖性方式诱导细胞凋亡。结论 EPA 显示出作为食管癌新疗法的潜力。
BACKGROUND Eicosapentaenoic acid (EPA) suppresses the proliferation of cell lines derived from colon, pancreatic, breast and other cancers. Few reports have described the effect of EPA on esophageal cancer cell lines. MATERIALS AND METHODS We investigated the effect of EPA on the proliferation of the esophageal squamous cell carcinoma cell lines TE11 and KYSE180 with a WST-1 assay. Apoptosis was evaluated with a DNA fragmentation assay. Levels of apoptosis-related proteins (caspase-3, -7, -9 and poly (ADP-ribose) polymerase (PARP)) and cleaved caspase-3, -7, -9 and PARP were evaluated by western blot analysis. RESULTS After exposure to EPA for 24 h, KYSE180 and TE11 cell proliferation was suppressed in a dose-dependent manner (p<0.05). In addition, caspase -3, -7, -9 and PARP were activated. EPA (0.1 μM, 1 μM, 10 μM) induced apoptosis in a dose-dependent manner, as detected by the DNA fragmentation assay. CONCLUSION EPA shows potential as a new treatment for esophageal cancer.