Dehydration prompts increased activity and blood feeding by mosquitoes.
Dehydration prompts increased activity and blood feeding by mosquitoes.
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DOI：
10.1038/s41598-018-24893-z
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发表时间：
2018-05-01
影响因子：
4.6
通讯作者：
Benoit JB
中科院分区：
文献类型：
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作者：
Hagan RW;Didion EM;Rosselot AE;Holmes CJ;Siler SC;Rosendale AJ;Hendershot JM;Elliot KSB;Jennings EC;Nine GA;Perez PL;Rizlallah AE;Watanabe M;Romick-Rosendale LE;Xiao Y;Rasgon JL;Benoit JB
目前对蚊子脱水反应的认识依赖于对特定反应的研究，但最终未能提供一个全面的蚊子生物学观点。在这里，我们研究了与脱水相关的蚊子生物学的潜在变化。具体来说，我们表明脱水会增加北方家蚊（库蚊）的血液摄取量，这是更高的活动和更大的倾向落在宿主身上的结果。埃及伊蚊和四方按蚊也有类似的观察结果。RNA-seq和代谢组学分析显示，脱水后与碳水化合物代谢相关的因素发生了改变，特别是海藻糖的分解。RNA干扰对库蚊海藻糖分解的抑制降低了与低水合水平相关的表型。最后，对库蚊的中观研究证实，在生态相关条件下，脱水的蚊子更有可能宿主饲料。疾病模型表明，脱水可能会增强病毒传播。因此，这种由脱水引起的供血增加很可能有规律地发生，并在水分不足时加剧。
Current insights into the mosquito dehydration response rely on studies that examine specific responses but ultimately fail to provide an encompassing view of mosquito biology. Here, we examined underlying changes in the biology of mosquitoes associated with dehydration. Specifically, we show that dehydration increases blood feeding in the northern house mosquito, Culex pipiens, which was the result of both higher activity and a greater tendency to land on a host. Similar observations were noted for Aedes aegypti and Anopheles quadrimaculatus. RNA-seq and metabolome analyses in C. pipiens following dehydration revealed that factors associated with carbohydrate metabolism are altered, specifically the breakdown of trehalose. Suppression of trehalose breakdown in C. pipiens by RNA interference reduced phenotypes associated with lower hydration levels. Lastly, mesocosm studies for C. pipiens confirmed that dehydrated mosquitoes were more likely to host feed under ecologically relevant conditions. Disease modeling indicates dehydration bouts will likely enhance viral transmission. This dehydration-induced increase in blood feeding is therefore likely to occur regularly and intensify during periods when availability of water is low.