Natural Phosphatidylcholine Is Actively Translocated across the Plasma Membrane to the Surface of Mammalian Cells*
Natural Phosphatidylcholine Is Actively Translocated across the Plasma Membrane to the Surface of Mammalian Cells*
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天然磷脂酰胆碱主动穿过质膜转移到哺乳动物细胞的表面*
DOI：
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发表时间：
2004
影响因子：
4.8
通讯作者：
G. van Meer
中科院分区：
文献类型：
--
作者：
Nanette Kälin;J. Fernandes;S. Hrafnsdóttir;G. van Meer
真核细胞的细胞表面富含胆碱磷脂，而氨基磷脂通过一种或多种P型ATP酶的活性浓缩在质膜的胞质侧。由于内源性脂质的测定本身就很复杂，因此大多数情况下使用短链脂质类似物来研究脂质转运。在本论文中，我们优化了两个独立的测定天然磷脂酰胆碱（PC）的易位到细胞表面的基础上的水解外叶磷酸甘油脂由外源性磷脂酶A2和交换外叶PC的转移蛋白。我们报告说，PC到达细胞表面的情况下，囊泡交通的途径，涉及跨质膜易位。在红细胞中，PC，这是在质膜内标记的易位到细胞表面的半衰期为30分钟。这种易位可能是由ATP酶介导的，因为它需要ATP和钒酸盐敏感。格列本脲（一种ATP结合盒转运蛋白的抑制剂）对PC易位的抑制作用及其在Abcb 1a/1b和Abcb 4基因敲除小鼠红细胞中的减少表明ATP结合盒转运蛋白参与了天然PC细胞表面易位。与磷脂酰丝氨酸的快速内向易位相比，PC的外向易位对于质膜中整体不对称磷脂组织的相对重要性仍有待确定。
The cell surface of eukaryotic cells is enriched in choline phospholipids, whereas the aminophospholipids are concentrated at the cytosolic side of the plasma membrane by the activity of one or more P-type ATPases. Lipid translocation has been investigated mostly by using short chain lipid analogs because assays for endogenous lipids are inherently complicated. In the present paper, we optimized two independent assays for the translocation of natural phosphatidylcholine (PC) to the cell surface based on the hydrolysis of outer leaflet phosphoglycerolipids by exogenous phospholipase A2 and the exchange of outer leaflet PC by a transfer protein. We report that PC reached the cell surface in the absence of vesicular traffic by a pathway that involved translocation across the plasma membrane. In erythrocytes, PC that was labeled at the inside of the plasma membrane was translocated to the cell surface with a half-time of 30 min. This translocation was probably mediated by an ATPase, because it required ATP and was vanadate-sensitive. The inhibition of PC translocation by glibenclamide, an inhibitor of various ATP binding cassette transporters, and its reduction in erythrocytes from both Abcb1a/1b and Abcb4 knockout mice, suggest the involvement of ATP binding cassette transporters in natural PC cell surface translocation. The relative importance of the outward translocation of PC as compared with the well characterized fast inward translocation of phosphatidylserine for the overall asymmetric phospholipid organization in plasma membranes remains to be established.
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DOI：
10.1016/s0021-9258(18)41791-7
发表时间：
1992-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Connor;C. Pak;R. Zwaal;A. Schroit
通讯作者：
J. Connor;C. Pak;R. Zwaal;A. Schroit
DOI：
10.1016/s0021-9258(18)42330-7
发表时间：
1992-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Arduini;G. Mancinelli;G. Radatti;S. Dottori;F. Molajoni;R. Ramsay
通讯作者：
A. Arduini;G. Mancinelli;G. Radatti;S. Dottori;F. Molajoni;R. Ramsay
已建立的细胞系中荧光磷脂酰胆碱类似物的细胞内转运的差异。
影响因子：
4
作者：
Sleight,RG;Abanto,MN
通讯作者：
Abanto,MN
影响因子：
2.9
作者：
Fielding, PE;Nagao, K;Fielding, CJ
通讯作者：
Fielding, CJ
人红细胞膜中的肉碱棕榈酰转移酶。
DOI：
10.1042/bj2750685
发表时间：
1991
期刊：
The Biochemical journal
影响因子：
--
作者：
Ramsay,RR;Mancinelli,G;Arduini,A
通讯作者：
Arduini,A