Food allergy promotes a Th2/Th17 response that drives house dust mite-induced allergic airway inflammation in humanized mice
Food allergy promotes a Th2/Th17 response that drives house dust mite-induced allergic airway inflammation in humanized mice
复制标题
食物过敏促进 Th2/Th17 反应，从而驱动人源化小鼠中屋尘螨诱发的过敏性气道炎症
DOI：
10.1111/cei.13504
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Li D.
中科院分区：
文献类型：
--
作者：
Wang B.;Hu J.;Liu Y.;Liu Q.;Li D.
食物过敏与变应性哮喘发病风险增加有关，但对身体不同部位不同过敏原致敏之间的确切相互作用尚未完全了解。本研究的目的是开发一种新的混合食物和呼吸道过敏的人源化小鼠模型，重现人类过敏反应，并更清楚地了解食物过敏对哮喘的影响。将来自花生和屋尘螨（HDM）过敏供体的外周血单个核细胞（PBMC）转移到免疫缺陷小鼠中，暴露于花生并对其进行攻击。在花生暴露和攻击之间，小鼠鼻内接受HDM治疗。分析过敏参数。过敏原特异性免疫球蛋白（IG）E只能在小鼠外周血单个核细胞（PBMC）加过敏原治疗中测量。先前的花生暴露增加了IgE水平、组胺释放、支气管高反应性和肺部炎症。当存在这两种过敏时，炎症细胞向气道的募集会加剧，这与辅助性T细胞2型（Th 2）/Th 17细胞因子分泌的增强有关。在这种人源化模型中，先前的花生暴露放大了过敏性哮喘，这可能有助于理解过敏个体中发生多敏化的潜在免疫学机制和评估治疗干预措施。
Food allergy is related to increasing risk of the development of allergic asthma, but the precise interplay between sensitization to different allergens in different compartments of the body is not fully understood. The aim of this study was to develop a novel humanized murine model of mixed food and respiratory allergy that recapitulates the human anaphylactic response and to more clearly understand the impact of food allergies on asthma. Immunodeficient mice transferred with peripheral blood mononuclear cells (PBMCs) from donors with peanut and house dust mite (HDM) allergy were exposed and challenged to peanut. Between peanut exposure and challenge, mice were intranasally treated to HDM. Allergic parameters were analyzed. Allergen-specific immunoglobulin (Ig)E in sera could only be measured in mice treated with peripheral blood mononuclear cells (PBMCs) plus allergen. A preceding peanut exposure increased IgE levels, histamine release, bronchial hyper-responsiveness and lung inflammation. Recruitment of inflammatory cells to the airways was aggravated associated with an enhanced T helper type 2 (Th2)/Th17 cytokine secretion when the two allergies were present. A preceding peanut exposure amplifies allergic asthma in this humanized model, which may contribute to the understanding of underlying immunological mechanism of polysensitization occurring in allergic individuals and evaluation of therapeutic interventions.