Metabolome strategy against Edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias
Metabolome strategy against Edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias
复制标题
通过葡萄糖增强代谢调节来对抗罗非鱼迟缓爱德华氏菌感染的代谢组策略
DOI：
10.1016/j.fsi.2015.06.004
复制
发表时间：
2015-08-01
影响因子：
4.7
通讯作者：
Li, Hui
中科院分区：
文献类型：
--
作者：
Peng, Bo;Ma, Yan-mei;Li, Hui
迟缓爱德华氏菌（Edwardsiellatarda）是造成鱼类病害的主要病原菌之一，给鱼类生产造成了巨大的经济损失。然而，针对病原体的代谢策略仍然未被探索。本研究采用基于GC-MS的代谢组学方法，研究了亚致死剂量E.迟到。死亡组和存活组之间的代谢差异允许识别感染期间的关键途径和关键代谢物。更重要的是，这些代谢产物可能通过调节与生存相关的代谢物组来增强抗感染能力。我们的数据表明，罗非鱼产生两种不同的策略，生存代谢组和死亡代谢组，遇到EIB 202感染，导致差异输出的生存和死亡。葡萄糖是最关键的生物标志物，分别在存活组和死亡组中上调和下调。注射或口服外源性葡萄糖可增强宿主抵抗EIB 202感染的能力，提高宿主的存活率。这些发现强调了宿主采取代谢策略来科普细菌感染，从中可以鉴定出关键的生物标志物来增强代谢策略。(C)2015爱思唯尔有限公司版权所有。
Edwardsiella tarda causes fish disease and great economic loss. However, metabolic strategy against the pathogen remains unexplored. In the present study, GC MS based metabolomics was used to investigate the metabolic profile from tilapias infected by sublethal dose of E. tarda. The metabolic differences between the dying group and survival group allow the identification of key pathways and crucial metabolites during infections. More importantly, those metabolites may modulate the survival-related metabolome to enhance the anti-infective ability. Our data showed that tilapias generated two different strategies, survival-metabolome and death-metabolome, to encounter EIB202 infection, leading to differential outputs of the survival and dying. Glucose was the most crucial biomarker, which was upregulated and downregulated in the survival and dying groups, respectively. Exogenous glucose by injection or oral administration enhanced hosts' ability against EIB202 infection and increased the chances of survival. These findings highlight that host mounts the metabolic strategy to cope with bacterial infection, from which crucial biomarkers may be identified to enhance the metabolic strategy. (C) 2015 Elsevier Ltd. All rights reserved.