Variation in the electrical properties of cultured human proximal tubule cells.
Variation in the electrical properties of cultured human proximal tubule cells.
复制标题
培养的人近曲小管细胞电特性的变化。
DOI：
10.1007/bf02633984
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Sens,DA
中科院分区：
文献类型：
--
作者：
Todd,JH;Sens,MA;Hazen-Martin,DJ;Bylander,JE;Smyth,BJ;Sens,DA
单层人近端小管（HPT）细胞，当生长在可渗透的支持和安装在Ussing室，自发显示跨上皮电位差（PD），短路电流（Isc），和跨上皮比电阻（RT）。这些电参数用于确定人近端小管细胞培养物的独立分离株之间的异质性程度。对17个独立的细胞分离株进行了评估，共进行了260次自发电特性测定。平均而言，这些细胞单层显示出1.5 ± 0.1 mV的顶端负PD、2.7 ± 0.2 µA/cm 2的Isc和480 ± 19 ohms × cm 2的RT。然而，每个独立的细胞分离株显示的电气值在一个狭窄的范围内，在某些情况下，允许分离株相互区分。还评估了单个分离株的Na-偶联葡萄糖转运、Na+，K+-ATP酶活性、甲状旁腺激素（PTH）对cAMP的刺激、毛喉素对Isc的刺激和哇巴因抑制。除Na+，K+-ATP酶活性和Isc之间的强相关性，这些参数，相反，电气性能，被认为是一致的，并没有揭示菌株之间的区别。HPT细胞培养物似乎始终保留了近端小管分化的重要特征，同时保持了变异性，如电特性所示，这可能是从随机群体中分离的细胞所预期的。
Monolayers of human proximal tubule (HPT) cells, when grown on permeable supports and mounted in Ussing chambers, spontaneously display a transepithelial potential difference (PD), short-circuit current (Isc), and transepithelial specific resistance (RT). These electrical parameters were used to determine the degree of heterogeneity among independent isolates of human proximal tubule cell cultures. Seventeen independent isolates of cells were assessed, totaling 260 individual determinations of spontaneous electrical properties. On average, these cell monolayers displayed an apicalnegative PD of 1.5 ± 0.1 mV, an Isc of 2.7 ± 0.2 µA/cm2, and an RTof 480 ± 19 ohms × cm2. Each independent cell isolate, however, displayed electrical values within a narrow range, in some cases allowing isolates to be distinguished from one another. The individual isolates were also assessed for Na-coupled glucose transport, Na+,K+-ATPase activity, cAMP stimulation by parathyroid hormone (PTH), forskolin stimulation of Isc, and ouabain inhibition. With the exception of a strong correlation between Na+,K+-ATPase activity and Isc, these parameters, in contrast to electrical properties, were found to be consistent and did not reveal distinctions among the isolates. HPT cell cultures seem to consistently retain important features of proximal tubule differentiation while maintaining the variability, as demonstrated by electrical properties, that might be expected of cells isolated from a random population.