PICKLE acts during germination to repress expression of embryonic traits
PICKLE acts during germination to repress expression of embryonic traits
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DOI：
10.1111/j.1365-313x.2005.02602.x
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发表时间：
2005-12-01
期刊：
影响因子：
7.2
通讯作者：
Ogas, J
中科院分区：
文献类型：
--
作者：
Li, HC;Chuang, K;Ogas, J
PICKLE（PKL）编码CHD3染色质重塑因子，其在拟南芥生长和发育中起多种作用。先前对表现出PKL依赖性调节的基因表达的分析表明，PKL在萌发过程中抑制胚胎性状的表达。在这项研究中，我们检查PKL蛋白的表达，以调查PKL何时何地起作用，以调节发育。PKL：eGFP翻译融合优先定位于细胞核中，这与PKL作为染色质重塑因子的拟议作用一致。类固醇诱导型PKL [PKL与糖皮质激素受体（PKL：GR）的融合物]用于检查PKL何时抑制胚胎性状的表达。我们发现，PKL：GR在萌发过程中的激活足以抑制胚胎性状的表达在初级根的PKL幼苗，而激活PKL：GR萌发后几乎没有影响。与此相反，我们观察到，PKL需要不断发芽后抑制PHERES 1，I型MADS盒基因，通常在早期胚胎发生在野生型植物中表达的表达。因此，PKL在发育过程中的多个点发挥作用，以调节拟南芥中的基因表达模式。
PICKLE (PKL) codes for a CHD3 chromatin remodeling factor that plays multiple roles in Arabidopsis growth and development. Previous analysis of the expression of genes that exhibit PKL-dependent regulation suggested that PKL acts during germination to repress expression of embryonic traits. In this study, we examined the expression of PKL protein to investigate when and where PKL acts to regulate development. A PKL:eGFP translational fusion is preferentially localized in the nucleus of cells, consistent with the proposed role for PKL as a chromatin remodeling factor. A steroid-inducible version of PKL [a fusion of PKL to the glucocorticoid receptor (PKL:GR)] was used to examine when PKL acts to repress expression of embryonic traits. We found that activation of PKL:GR during germination was sufficient to repress expression of embryonic traits in the primary roots of pkl seedlings, whereas activation of PKL:GR after germination had little effect. In contrast, we observed that PKL is required continuously after germination to repress expression of PHERES1, a type I MADS box gene that is normally expressed during early embryogenesis in wild-type plants. Thus, PKL acts at multiple points during development to regulate patterns of gene expression in Arabidopsis.